Functional genomics

Now that most genomes of model organisms have been sequenced and annotated in detail, reverse genetics approaches are being used to experimentally determine the function of genes encoded by genomes. Most gene functions up until today are largely based on bioinformatics-based predictions. However, those that have been experimentally analysed, especially regulatory genes, reveal a complex picture of genes having multiple and often overlapping functions. In this complex framework, the course intends to provide the students with deep knowledge about the latest technologies and genome wide approaches to study gene functions in model organisms and to unravel complex regulatory pathways.

Students will become familiar with approaches used to study gene functions using state-of-the-art genomics, transcriptomics and proteomics approaches. The course commences with genome analysis and reverse genetics approaches applied to study gene functions. Subsequently, transcriptomics and protein analysis will be used to study transcription factors that control regulatory pathways. Students will gain competences in understanding the different methodologies used for gene function analysis in complex biological systems.
BY the end of the course, students will be able to design experimental strategies to study gene functions in prokaryotic and eukaryotic model organisms.