Biomolecular methods laboratory

Over the last few years, research has faced an impressive advancement in experimental methods. This course intends to expose the students to a large number of techniques in order to build a solid knowledge on the wide variety of available experimental tools. The course aims to equip students with a detailed understanding and knowledge of the molecular principles underlying the advanced techniques currently used in research laboratories. Students will acquire a deep knowledge on the molecular strategies adopted to purify and sequence nucleic acids. The course will provide the theoretical principles on which the techniques discussed in class are based, and the practical and technical details on the main biochemical techniques such as electrophoresis, chromatography and in vitro techniques that use antibodies. Students will also develop a better understanding of the experimental strategies applied to genome manipulation.

Students will benefit from this course by becoming competent in identifying the most appropriate techniques to use for their research purposes during their Master-thesis internship.
Exposing the students to a large number of techniques, currently used in research, will help them to build a solid background toward the most basic methodological tools, acquiring the competence to identify the most appropriate experimental strategy to use for their research purposes. Students will learn both the theoretical principles on which the techniques discussed in class are based, and the practical and technical details on the main biochemical techniques such as electrophoresis, chromatography and in vitro techniques that use antibodies. Students will also develop a better understanding of the experimental strategies applied to genome manipulation.