Biomolecular methods laboratory
A.A. 2024/2025
Obiettivi formativi
Over the last few years, research has faced an impressive advancement in experimental methods. This course intends to expose the students to a large number of techniques in order to build a solid knowledge on the wide variety of available experimental tools. The course aims to equip students with a detailed understanding and knowledge of the molecular principles underlying the advanced techniques currently used in research laboratories. Students will acquire a deep knowledge on the molecular strategies adopted to purify and sequence nucleic acids. The course will provide the theoretical principles on which the techniques discussed in class are based, and the practical and technical details on the main biochemical techniques such as electrophoresis, chromatography and in vitro techniques that use antibodies. Students will also develop a better understanding of the experimental strategies applied to genome manipulation.
Risultati apprendimento attesi
Students will benefit from this course by becoming competent in identifying the most appropriate techniques to use for their research purposes during their Master-thesis internship.
Exposing the students to a large number of techniques, currently used in research, will help them to build a solid background toward the most basic methodological tools, acquiring the competence to identify the most appropriate experimental strategy to use for their research purposes. Students will learn both the theoretical principles on which the techniques discussed in class are based, and the practical and technical details on the main biochemical techniques such as electrophoresis, chromatography and in vitro techniques that use antibodies. Students will also develop a better understanding of the experimental strategies applied to genome manipulation.
Exposing the students to a large number of techniques, currently used in research, will help them to build a solid background toward the most basic methodological tools, acquiring the competence to identify the most appropriate experimental strategy to use for their research purposes. Students will learn both the theoretical principles on which the techniques discussed in class are based, and the practical and technical details on the main biochemical techniques such as electrophoresis, chromatography and in vitro techniques that use antibodies. Students will also develop a better understanding of the experimental strategies applied to genome manipulation.
Periodo: annuale
Modalità di valutazione: Esame
Giudizio di valutazione: voto verbalizzato in trentesimi
Corso singolo
Questo insegnamento può essere seguito come corso singolo.
Programma e organizzazione didattica
Edizione unica
Responsabile
Periodo
annuale
Programma
Course syllabus
The course aims to equip students with a detailed understanding and knowledge of the molecular principles underlying the advanced techniques currently used in research laboratories, exposing students to a large number of techniques in order to build a solid knowledge on the wide variety of available experimental tools. Students will acquire a deep knowledge on the molecular strategies adopted to purify and sequence nucleic acids, the technical details on the main biochemical techniques such as electrophoresis, chromatography and in vitro techniques that use antibodies. Students will also develop a better understanding of the experimental strategies applied to genome manipulation.
Manipulation of cell cultures (transfection) and animal models (transgenic animals) will also be part of the course.
The course aims to equip students with a detailed understanding and knowledge of the molecular principles underlying the advanced techniques currently used in research laboratories, exposing students to a large number of techniques in order to build a solid knowledge on the wide variety of available experimental tools. Students will acquire a deep knowledge on the molecular strategies adopted to purify and sequence nucleic acids, the technical details on the main biochemical techniques such as electrophoresis, chromatography and in vitro techniques that use antibodies. Students will also develop a better understanding of the experimental strategies applied to genome manipulation.
Manipulation of cell cultures (transfection) and animal models (transgenic animals) will also be part of the course.
Prerequisiti
Prerequisites for admission
A good knowledge of molecular biology, biochemistry and genetics is required.
A good knowledge of molecular biology, biochemistry and genetics is required.
Metodi didattici
Teaching methods
Lessons will be based on powerpoint presentations and interactive discussions. Students will be stimulated to critically analyze the techniques that will be explained and try to understand which experimental strategies to use in different cases.
Lessons will be based on powerpoint presentations and interactive discussions. Students will be stimulated to critically analyze the techniques that will be explained and try to understand which experimental strategies to use in different cases.
Materiale di riferimento
Teaching Resources
Powerpoint presentations and lists of reference books will be made available through the Ariel website.
It is important to stress that the course is mainly based on the more used techniques in research laboratories.
Powerpoint presentations and lists of reference books will be made available through the Ariel website.
It is important to stress that the course is mainly based on the more used techniques in research laboratories.
Modalità di verifica dell’apprendimento e criteri di valutazione
Assessment methods and Criteria
Two workshops will be held (one in May and one in September) during which students will select an innovative, methodological-based paper that, upon approval of the teachers, may be presented in the form of a Powerpoint presentation at the workshop. The workshop grade contributes to 30% of the final grade. A final, written laboratory report consisting of a short abstract and the Material and Methods section of the Master's Thesis, together with short summaries describing two seminars attended by the student, will form the bases for the final oral exam, that contributes to the remaining 70% of the final grade. Those who do not give a Workshop presentation can obtain a maximum final grade of 27.
Two workshops will be held (one in May and one in September) during which students will select an innovative, methodological-based paper that, upon approval of the teachers, may be presented in the form of a Powerpoint presentation at the workshop. The workshop grade contributes to 30% of the final grade. A final, written laboratory report consisting of a short abstract and the Material and Methods section of the Master's Thesis, together with short summaries describing two seminars attended by the student, will form the bases for the final oral exam, that contributes to the remaining 70% of the final grade. Those who do not give a Workshop presentation can obtain a maximum final grade of 27.
BIO/10 - BIOCHIMICA - CFU: 3
BIO/11 - BIOLOGIA MOLECOLARE - CFU: 3
BIO/18 - GENETICA - CFU: 3
BIO/11 - BIOLOGIA MOLECOLARE - CFU: 3
BIO/18 - GENETICA - CFU: 3
Lezioni: 24 ore
Lezioni (seminario): 48 ore
Lezioni (seminario): 48 ore
Siti didattici
Docente/i
Ricevimento:
martedi' 11.30-12.30 per appuntamento via email
7C
Ricevimento:
Su richiesta
Torre 5B, Dip. Bioscienze, Via celoria 26, 20133