Experimental Medicine

Dottorati
Doctoral programme (PhD)
A.Y. 2024/2025
Study area
Medicine and Healthcare
Doctoral programme (PhD)
3
Years
Dipartimento di Biotecnologie mediche e medicina traslazionale - Via L. Vanvitelli, 32 - 20129 Milano
English
PhD Coordinator
Experimental medicine develops and applies technology and biological models for studying pathogenic mechanisms in human disease. It involves various disciplines (biochemistry, biology, molecular biology, anatomy, physiology, pathology, immunology, genetics and microbiology/virology) that are providing a rapidly growing understanding of the mechanisms that enable the development of pathological processes. The course aims to acquaint students with the technologies and research methodologies in the area of biomedicine and biotechnology based on in vitro and in vivo experimental models and '-omic' approaches, and on their application to the study of cellular and molecular mechanisms involved in the pathogenesis of human disease. The final objective is the formation of high-level scientists able to combine an understanding of biological processes and pathogenic mechanisms through the application of advanced experimental techniques. The programme's specific objectives are:
1) develop the ability to define problems and devise experiments to solve them in accordance with scientific standards;
2) study biochemical, molecular and cellular mechanisms in physiopathology using advanced technologies;
3) develop the ability to critically assess scientific literature;
4) provide solid, up-to-date knowledge in the specific field;
5) teach how to conduct independent research, and inform on related ethical implications.
Tutte le classi di laurea magistrale - All classes of master's degree
Dipartimento di Biotecnologie mediche e medicina traslazionale - Via L. Vanvitelli, 32 - 20129 Milano
  • Degree course coordinator: Nicoletta Landsberger
    [email protected]
  • Main offices
    Dipartimento di Biotecnologie mediche e medicina traslazionale - Via L. Vanvitelli, 32 - 20129 Milano
  • Degree course website
    http://d-memphd.unimi.it/
Title Professor(s)
Toward a Personalized Therapy in Neurodevelopmental Pathologies
Requirements: Conoscenza delle principali tecniche elettrofisiologiche per lo studio della comunicazione neuronale, uso dei modelli animali per sperimentazione in vitro e in vivo, conoscenze delle tecniche di imaging
Study of fear memory consolidation pathways in Post-Traumatic Stress Syndrome (PTSD) and their potential implications in disease prevention
Requirements: Basic experience working in vivo with experimental preclinical models in particular knowledge with behavioral analyses and pharmacological treatments. Good expertise in molecular biology approaches will be considered as a preferential qualification.
E. BATTAGLIOLI
F. S. RUSCONI
Caratterizzazione e modulazione dei neuroni “immaturi”: una potenziale risorsa di cellule non proliferanti per la plasticità della corteccia cerebrale murina e umana
Requirements: Sono invitati a partecipare studenti altamente motivati con un forte interesse nella ricerca biomedica di base e traslazionale in ambito neuroscienze e medicina rigeneraritiva. Forte attitudine al lavoro di gruppo
Decifering of the complex genetic basis of GnRH-secreting neuron developmental defects
Requirements: Eventual experience on endocrine regulatory mechanisms of reproduction, whole exome sequencing technique and analysis, molecular biology techniques for gene expression studies, zebrafish model and pluripotent stem cells
Development of organoid-based models to study the impact of nanocellulose upon gastro-intestinal exposure: assessing immune responses at epithelial barriers in healthy and disease conditions
Requirements: The candidate has to demonstrate experience in the main techniques of cell biology and molecular biology as well as in cultures of mammalian cells. Experience in cultivating induced pluripotent stem (iPS) cells, knowledge of immunology and flow cytometry will be considered as an advantage
M. PAULIS (IRGB-CNR)
Co-culture of endothelial cells and osteoblasts in microgravity: effects on osteoblast activity
Requirements: Cell culture, Western Blotting, Nucleic acids extraction, Real-Time PCR, gene silencing and editing.
ELUCIDATING THE MECHANISM OF RESISTANCE TO CAR-T THERAPY IN LARGE B-CELL LYMPHOMAS
Requirements: experience working with multimodal datasets, including NGS data, and the interest and ability to manipulate and analyze complex data structures; interest or proficiency in statistical programming languages such as R or Python; interest in translational research, hemathology and cell therapies; enthusiasm and persistence in the application of analytical methods to complex biological problems.
C. CARNITI (Istituto Nazionale dei Tumori)
Addressing autoimmunity and immune deficiency in thymomas, at the crossroad between cancer immunology and immune dysfunction
Requirements: Basic experience in cell culture techniques; basic experience in flow cytometry, molecular biology and related computational analytical approaches; willingness to spend periods abroad for training and collaborations
P. ZUCALI (HUNIMED)
Dipeptidyl Peptidase-4 (DPP4) inhibition: role in obesity-induced inflammation and tissue injury
Requirements: knowledge of the main basic methods in cellular and molecular biology (cell culture techniques, gene and protein expression analysis), biochemical assays for the evaluation of biomarkers in biological fluids, histology & immunohistochemistry methods
Multi-omics analysis of iPS-derived neurons in 2D and 3D cultures for the study of Congenital Central Hypoventilation Syndrome
Requirements: Basic cellular and molecular biology techniques (PCR, RNA extraction, RT-qPCR, western blot), mammalian cell culture and immunofluorescence. iPS culture will be considered a preferential qualification
E. PISCITELLI (Istituto di Tecnologie Biomediche - CNR)
Analysis of GABAergic circuitry in Pcdh19 mouse model of Developmental and epileptic encephalopathy 9 (DEE9)
M. PASSAFARO (CNR-Neuroscience Institute)
Study of astrocyte-derived extracellular vesicles in Rett syndrome to identify novel therapeutic approaches.
Requirements: Experience in molecular biology and cell cultures
RNA therapies for the treatment of neurodevelopmental disorders
Requirements: Experience in molecular biology and cell cultures
New mechanisms controlling macrophages functions: role of Ms4a proteins
Requirements: Basic cellular and molecular biology; biochemical competences. Possible (not mandatory) in vivo experience
Identification of immune profiles in pancreatic cancer by multidimensional tissue analysis.
Requirements: Previous experience in tissue staining and analysis of immune cells
Phenotypic and molecular characterization of innate lymphoid cells in Myelodisplastic syndromes: towards the comprehension of their role in disease etiology and prognosis
Requirements: Basic training in immunology laboratory and good organization skills. Previous experience in flow cytometry and cell biology are a plus
Involvement of microglial cells in the etiology, neurobiology and treatment of stress-related psychiatric disorders
Requirements: Expertize in gene and protein expression analyses, cell culture
Decoding the Molecular Drivers of Cerebral Cavernous Malformations
M. TROVATO (IFOM)
Advanced understanding of Thyroid Hormone action in brain and heart using zebrafish model and induced pluripotent stem cells (iPSC)
Requirements: Experience in molecular biology and cell cultures
F. MARELLI (IRCCS Istituto Auxologico Italiano)
G. RURALE (IRCCS Istituto Auxologico Italiano)
Manipulation of insulin receptor (IR) alternative splicing as a novel therapeutic strategy to block IGF2 autocrine proliferative loop in adrenocortical carcinoma (ACC)
Requirements: Cell cultures; cell transfection and silencing; protein detection (western blot, co-/immunoprecipitation); nucleic acid extraction, PCR.
R. CATALANO
In vitro and in vivo (zebrafish) study on the efficacy of histone deacetylase (HDAC) inhibition for the treatments of glioblastoma
Requirements: Expertise in cellular and molecular biology. Preferred but not mandatory, experience in zebrafish and/or cellular models.
In vitro and in vivo omics approaches for studying the molecular mechanisms of human diseases, with a particular focus on glioblastoma.
Requirements: Expertise in cellular and molecular biology. Preferred but not mandatory, experience in zebrafish and/or cellular models
Tissue microbiota remodeling during lung cancer progression: a fuel for immunosuppression
The Molecular Mechanisms of Obesity driven to Cardiac Remodeling
Requirements: Basic practice in molecular biology: - RT-PCR - RNA/DNA extraction - Tissue Protein extraction - 2D Cell culture management
Adipo-NETwork: inside the crosstalk between adipose cells and gastroenteropancreatic neuroendocrine tumors (GEP-NETs)
Requirements: Strong interest for basic and translational research.
G. GAUDENZI (Istituto Auxologico Italiano IRCCS)
In vitro and in vivo (zebrafish) study on the efficacy of histone deacetylase (HDAC) inhibition for the treatments of glioblastoma
Requirements: Expertise in cellular and molecular biology. Preferred but not mandatory, experience in zebrafish and/or cellular models.

Enrolment

Places available: 12

Call for applications

Please refer to the call for admission test dates and contents, and how to register.

Application for admission: from 29/05/2024 to 27/06/2024

Read the Call


Attachments and documents

Attachments to the call

Extension

Qualifications assessment criteria

Scores and exam schedule

Notice of interview and enrolment dates