Omics Approaches: from Research to Therapy
A.Y. 2024/2025
Learning objectives
The teaching aims to provide students with the scientific knowledge contributing to the formation of a basic culture on omics technologies and the learning of the language to be used. The purpose of the teaching is to enable students to understand the fundamental logic in the use of omics methodologies, to know the theoretical and practical bases and principles underlying the different omics techniques, the main usage protocols both in sample preparation and instrumentation, and their control, and finally to provide the foundations for the management and analysis of results, also illustrating the possibility of integrating the data obtained with different omics methodologies. The educational objective of the teaching is to develop knowledge that allows students to address various scientific issues and to choose and apply the best omics methodologies to understand the biochemical functions in the main tissues or cellular models, also in relation to the most common pathologies.
This will set the stage for testing how the analysis of omics dataset could be used to improve patient stratification, the identification of novel pharmacological treatments and the evaluation of the safety and efficacy profile of biologics.
This will set the stage for testing how the analysis of omics dataset could be used to improve patient stratification, the identification of novel pharmacological treatments and the evaluation of the safety and efficacy profile of biologics.
Expected learning outcomes
The students' learning outcomes are assessed by the teachers through an oral and/or a written test to examine of the comprehension of the omics methodologies and their use based on the program carried out during the course. The examination of the students by the teachers will mainly focus on assessing the knowledge and understanding of omics techniques and evaluating the skills acquired by the student upon completion of the teaching itself. In particular, the language proficiency used by the student will be assessed, as well as the skills acquired to discriminate the choice of different omics methodologies and their potential integration to apply to various scientific issues.
Lesson period: Second semester
Assessment methods: Esame
Assessment result: voto verbalizzato in trentesimi
Single course
This course can be attended as a single course.
Course syllabus and organization
Single session
Responsible
Lesson period
Second semester
Course syllabus
The following topics will be discussed
- Introduction to Genomics
- An overview on Precision Medicine
- Large Scale Genome Sequencing Project
- Next Generation Sequencing Platforms
- Next Generation Sequencing libraries preparations and sequencing
- Next Generation Sequencing Applications: Whole Exome Sequencing (WES) and Whole Genome Sequencing (WGS)
- Next Generation Sequencing Applications: ATAC-seq, ChIP-seq, bulk-RNA-seq
- Spatial Transcriptomics
- Single Cell RNA-seq
- Introduction to proteomics
- Proteomic platform and instrumentation (high-resolution nanoLC-MS/MS system)
- Quantitative analysis in proteomics: Labelled and label-free approaches
- Imaging proteomics and applications
- Structural proteomics
- Introduction to metabolomics
- Metabolic platform: targeted vs untargeted metabolomics
- Lipidomics
- Fluxomics
- Introduction to bioinformatics
- Omics data integration
- Introduction to Genomics
- An overview on Precision Medicine
- Large Scale Genome Sequencing Project
- Next Generation Sequencing Platforms
- Next Generation Sequencing libraries preparations and sequencing
- Next Generation Sequencing Applications: Whole Exome Sequencing (WES) and Whole Genome Sequencing (WGS)
- Next Generation Sequencing Applications: ATAC-seq, ChIP-seq, bulk-RNA-seq
- Spatial Transcriptomics
- Single Cell RNA-seq
- Introduction to proteomics
- Proteomic platform and instrumentation (high-resolution nanoLC-MS/MS system)
- Quantitative analysis in proteomics: Labelled and label-free approaches
- Imaging proteomics and applications
- Structural proteomics
- Introduction to metabolomics
- Metabolic platform: targeted vs untargeted metabolomics
- Lipidomics
- Fluxomics
- Introduction to bioinformatics
- Omics data integration
Prerequisites for admission
Basic knowledge in Biochemistry, Applied Biochemistry, Molecular Biology, Immunology, Physiopathology and Pharmacology are strongly recommended.
Teaching methods
The course is composed of two teaching units: 1. "Omics technology and approaches" *24 h) and 2. "Omics strategy in Pharmacology" (16 h frontal teaching + 16 h for practical course). Both teaching units consist of lectures given by the Teachers and, occasionally, seminars by external experts on particular topics.
Teaching Resources
The iconographic materials shown during the lessons and bibliographic references can be downloaded from the Ariel portal.
Assessment methods and Criteria
The students' learning outcomes will be evaluated by written examination. Specifically, the written exam will consist of multiple-choice questions on the topics covered during the course for the two teaching units.
BIO/10 - BIOCHEMISTRY - University credits: 3
BIO/14 - PHARMACOLOGY - University credits: 3
BIO/14 - PHARMACOLOGY - University credits: 3
Practicals: 16 hours
Lessons: 40 hours
Lessons: 40 hours
Professor(s)