Biochemistry

A.Y. 2024/2025
8
Max ECTS
72
Overall hours
SSD
BIO/10
Language
Italian
Learning objectives
General aims of the course: (1) To introduce students to the molecular design of life, including structural and biological properties of the main constituents of organisms; (2) To describe the molecular basis of the main biological processes, with particular emphasis on the metabolic fate of the lipidic, proteic and glicidic components of food; (3) To detail molecular basis of energy production from the main food components under aerobic and anaerobic conditions; (4) To introduce students to the main molecular biology mechanisms, including DNA replication, RNA and protein synthesis; (5) To detail the structure-function relationship in proteins; (6) To introduce basic laboratory biochemical techniques.
Expected learning outcomes
1. Knowledge and understanding: the student, at the end of the course, will have to demonstrate to have basic knowledge relating to animal metabolism, with special emphasis on pets, in order to understand the structural/energetic/reserve role of the most relevant biomolecules. The student will also have to acquire knowledge relating to the structure and role of informational macromolecules (proteins and nucleic acids) as well as the main mechanisms involved in the regulation of biological processes. Theoretical-practical aspects of some basic biochemical laboratory techniques will also be presented.
2. Applying knowledge and understanding: At the end of the course, the student must be able to distinguish the main metabolic effects of the lipid, carbohydrate and protein components present in foods, with particular attention to their energy intake. This basic knowledge will be necessary to understand other aspects relating to animal welfare. Furthermore, the student must be able to understand some basic biochemical techniques useful in the analysis of biological samples.
3. Making judgments: the student must demonstrate the ability to critically argue the information acquired. During the course, students are encouraged to think about practical examples and group discussions.
4. Communication: the student must demonstrate the ability to use scientifically appropriate terminology, in particular concerning the terminology referring to the structure and function of the main biological molecules, metabolic processes, replication processes of DNA and the biosynthesis of RNA and proteins. The exercises and seminars will stimulate the ability to express oneself correctly and the ability to discuss scientifically with peers.
5. Lifelong learning skills: the student must demonstrate the ability to use the knowledge acquired to interpret structures of macromolecules and new metabolic pathways, searching scientific databases and critically organising new information.
Single course

This course can be attended as a single course.

Course syllabus and organization

Single session

Responsible
Lesson period
Second semester
Course syllabus
Theoretical lectures (tot.56 hours)
Amino acids, proteins and structural levels of proteins (3 hours)
Biological membranes and transport across membranes (4 hours)
Oxygen-transporting proteins (4 hours)
Collagen (1 hour)
Enzymes: kinetics and mechanisms of regulation, zymogens (4 hours)
Molecular mechanisms of digestion, absorption and transport of the main nutritionally relevant classes of compounds (6 hours)
Metabolism of carbohydrates: glycolysis, pentose phosphate pathway, metabolism of glycogen, gluoconeogenesis, biosynthesis of lactose (8 hours)
Metabolism of lipids: metabolism of fatty acids and ketone bodies, overview of metabolism of triacylglicerols, phosphoglycerids, sfingolipids, cholesterol and steroids (6 hours)
Krebs cycle and oxidative phosphorylation (4 hours)
Metabolism of amino acids: amino acid degradation and the urea cycle, overview of nitrogen fixation and amino acid biosynthesis (3 hours)
Overview of metabolism of nucleotides (2 hours)
Integration of metabolism and overview of hormonal control of metabolism (2 hours)
Vitamins (1 hour)
Molecular biology: structure and function of nucleic acids, genetic code, DNA replication, transcription and post-transcriptional modifications, protein synthesis and post-translational modifications (8 hours)


Exercitations and seminars (tot.16 h)
Calculations relating to the preparation of buffer solutions and samples, dilutions, quantity and concentration units of measurement (3 hours)
Principles and applications of spectrophotometric and colorimetric techniques for the measurement of metabolites and enzymes (3 hours)
Colorimetric detemination of glucose (1 hours)
Colorimetric detemination of proteins (Bradford) (3 hours)
Colorimetric detemination of alkaline phosphatase (2 hours)
Principles of mass spectrometry and its applications for analysis of the protein content in foods (4 hours)
Prerequisites for admission
In order to attend the course profitably the student should be endowed with basic knowledge of general, inorganic and organic chemistry. It is highly recommended to follow the course "Chemistry" held in the first semester. Alternatively, students can use any basic level chemistry textbook or on-line material.
Teaching methods
The course consists of classroom theoretical lectures supported by seminars and laboratory practice on main biochemical biochemical tecniques applied in analytical laboratories and specific topics of interest, with the aid of on-line scientific movies in order to describe the complex molecular events leading to nucleic acids and protein synthesis
Teaching Resources
Power Point slides on Ariel web-site (https://myariel.unimi.it)
One of the following textbooks:
D.L. Nelson M.M. Cox Introduzione alla biochimica di Lehninger (Zanichelli)
Campbell Farrell Biochimica (Edises)

Campbell Farrell Biochimica (Edises)
Assessment methods and Criteria
The examination consists of a written test consisting in 4 open-ended questions, at least one for each of the following general subject: metabolism and its control, general biochemistry, molecular biology and the structure of a nucleic acid. Students must complete the test in 120 minutes. The overall final score of the test, beside correctness, considers completeness, clarity, synthesis and use of proper terminology. Grade are in thirtieths.
Exams are in number of 7 in the Academic year and take place in the months of January, February, April June, July, September and October, with the frequency set by the teaching board. Results will be communicated by SIFA.
Tests will be the same for attending and non-attending students.
BIO/10 - BIOCHEMISTRY - University credits: 8
Practicals: 16 hours
Lessons: 56 hours
Shifts:
Turno
Professors: Negri Armando, Nonnis Simona
Professor(s)
Reception:
Monday 12.30-13.30
Department of Veterinary Medicine, Via dell'Università, 6 - 26900 Lodi, Italy