Basic Concepts of Molecular Pathology
A.Y. 2024/2025
Learning objectives
The course aims to provide students with instruments to understand the molecular bases of pathological disorders and practical skills related to molecular methodologies for diagnosis and research for the study of animal diseases.
Lectures focus on the most common molecular techniques and their application to the study and diagnosis of diseases.
During practical classes, students will acquire skills in molecular techniques for the study of proteins (Western blot, immunohistochemistry, flow cytometry) and nucleic acids (PCR and RT-PCR).
Lectures focus on the most common molecular techniques and their application to the study and diagnosis of diseases.
During practical classes, students will acquire skills in molecular techniques for the study of proteins (Western blot, immunohistochemistry, flow cytometry) and nucleic acids (PCR and RT-PCR).
Expected learning outcomes
At the end of the course, the student will acquire the fundamental knowledge of the molecular mechanisms of disorders and the basic techniques to investigate them.
Lesson period: Second semester
Assessment methods: Esame
Assessment result: voto verbalizzato in trentesimi
Single course
This course can be attended as a single course.
Course syllabus and organization
Single session
Responsible
Lesson period
Second semester
Course syllabus
The course will provide theoretical and practical knowledge of basic biomolecular methodologies useful for diagnostics and research for the study of animal diseases. During the theoretical hours, lectures are provided on the most common biomolecular techniques and their application to the study and diagnosis of diseases of companion animals and livestock.
Practicals
· Protein extraction, and protein quantification using Bradford, Western blot, and blu coomassie; (4 hours)
· mRNA extraction, retrotranscription, qPCR; (4 hours)
· Cellular biology: PBMC isolation and viability test; (4 hours)
· Drafting report on practical classes and collegial discussion (4 hours).
Practicals
· Protein extraction, and protein quantification using Bradford, Western blot, and blu coomassie; (4 hours)
· mRNA extraction, retrotranscription, qPCR; (4 hours)
· Cellular biology: PBMC isolation and viability test; (4 hours)
· Drafting report on practical classes and collegial discussion (4 hours).
Prerequisites for admission
Recommended: basic knowledge of general pathology, biochemistry, and immunology.
Teaching methods
-Classroom lectures (16 hours)
-Classroom practice (12 hours)
-collegial discussion (4 hours)
-Classroom practice (12 hours)
-collegial discussion (4 hours)
Teaching Resources
Teaching material will be posted on the ARIEL platform. Exam questions will be extracted only from the teaching material made available.
Consultation text: Robbin's and Cotran's Pathologic basis of Disease only to be considered as a suggestion for those students who would like to deepen their knowledge in general and special pathology. This book is a human pathology book.
Consultation text: Robbin's and Cotran's Pathologic basis of Disease only to be considered as a suggestion for those students who would like to deepen their knowledge in general and special pathology. This book is a human pathology book.
Assessment methods and Criteria
The examination consists of a written test with 32 questions (multiple choice and open answers) on the topic reported in the course slides and available on Moodle. Practical class reports are also evaluated and they contribute 33% (=1 CFU) of the final grade. The students who cannot attend the practical class will draft a report on the methodology of the practical class.
- Duration of the test (in the case of a written test): 120 minutes
- Assessment parameters: each answer is given a score = 1; in the assessment of open-ended answers the following assessment elements are adopted: knowledge of the subjects score=1; clarity of exposition score=0.5; and use of the appropriate terminology score=0,5.
- Type of assessment: grade in thirtieths
- Method of communicating assessment results (in the case of written tests): via SIFA and by email.
- Duration of the test (in the case of a written test): 120 minutes
- Assessment parameters: each answer is given a score = 1; in the assessment of open-ended answers the following assessment elements are adopted: knowledge of the subjects score=1; clarity of exposition score=0.5; and use of the appropriate terminology score=0,5.
- Type of assessment: grade in thirtieths
- Method of communicating assessment results (in the case of written tests): via SIFA and by email.
VET/03 - VETERINARY PATHOLOGY - University credits: 3
Practicals: 16 hours
Lessons: 16 hours
Lessons: 16 hours
Professor:
Lecchi Cristina
Shifts:
Turno
Professor:
Lecchi CristinaProfessor(s)
Reception:
upon appointment
Via dell'Università 6, Lodi