Veterinary General Pathology and Clinical Biochemistry

A.Y. 2024/2025
12
Max ECTS
112
Overall hours
SSD
BIO/12 VET/03
Language
Italian
Learning objectives
To provide knowledge about the pathogenic mechanisms responsible of the pathologic changes detectable in domestic and wild animals. Students will be also educated to select appropriate clinical biochemistry and molecular biology methods and to elaborate, interpret and understand the limitation of the test results.
Expected learning outcomes
Knowledge and understanding: the student will acquire knowledge on the etiopathogenesis of the main pathological and pathophysiological changes in animal tissues and organ systems and on the analytical techniques commonly used in veterinary clinical chemistry, biochemistry and molecular biology.
Ability to apply knowledge and understanding: the student will acquire skills to interpret the most common pathological and pathophysiological changes and the associated laboratory abnormalities, as well as to select the most appropriate biochemical and molecular methods to measure diagnostic markers of disease in biological samples of animal origin. The student will learn how to process an instrumental reading into the concentration of a diagnostic molecular marker, and on how to interpret the diagnostic value of a laboratory result, how to control the source of error of laboratory's tests and the principle of quality insurance.
Autonomy of judgment: the student will develop problem solving abilities and autonomy of judgment by tackling problems of diagnostic clinical pathology and biochemistry during practical exercises in laboratories (biochemistry, microscopy and computer labs). The ability to integrate notions taught in the different modules will also developed by proposing to small group of students clinical cases that they must evaluate and solve autonomously as regards the aspects of pathology, immunopathology and clinical biochemistry.
Communication skills: the student during the class discussion and practical laboratory exercises will learn how to present pathology and clinical biochemistry data with appropriate and updated terminology, consistent with the professionalism required by a veterinarian surgeon. Communication skills will be as well developed during collegial discussions of the diagnostic pathology and biochemistry matters of the clinical cases that the students previously faced by themselves.
Lifelong learning skills: the reasoning skills and the notions and methodologies acquired during the classes should allow the student to continue autonomously the study and the lifelong updating of his knowledge on tissue and organ pathological and pathophysiological alterations, as well as on the method of analysis of clinical biochemistry markers of disease, both during the university career and then as a veterinarian.
Single course

This course can be attended as a single course.

Course syllabus and organization

Single session

Responsible
Lesson period
Second semester
If neede, the lectures will be held online, through videolectures recorded and made available to the students or through lectures held online on a sinchronous platform. Practicals will be held online with examples of analytical methods, case discussion and practical tests
Prerequisites for admission
Attendance to lectures and practices of the three modules and passing the exams of propedeutical courses (barrier exam) of:
- Batteriologia, virologia e immunologia veterinaria
- Anatomia veterinaria sistematica e comparata 3
- Fisiologia ed endocrinologia degli animali domestici
- Fisiologia ed etologia degli animali domestici
Assessment methods and Criteria
There are 8 exam session, taking place in: January, February, April, June, July, September, October, November / December. Two extra exam sessions only for students enrolled in supplementary years take place on request in March and May.
To take the exam it is compulsory to register trough the SIFA online service (N.B.: registration closes 3 days before the date of the exam) and the propaedeutic exams must have been passed.

The examination takes place in a single session for the three modules of the integrated course and consists of a written exam of a maximum duration of 120 minutes.
The student who scores ≥ 18 points in all three modules passes the written test. A student who scores < 16 points in two or more modules is rejected. A student who scores 16 or 17 points in one module and ≥ 18 in the other two is required to take an oral test of the insufficient module in order to pass the integrated examination. The oral test consists of at least 3 questions per module of the integrated course being taken. Students who scored ≥ 18 in the three modules and wish to increase their written score may request the oral of one or more modules on a voluntary basis. The optional oral interview is devoted to critical discussion of the written score, evaluation of possible errors in evaluation, and questions on specific topics of the program. During the oral examination, it will be assessed as well whether the knowledge and ability to understand and apply the knowledge, the ability to criticise and make judgements, the ability to present the acquired notions with appropriate language consistent with the course subjects are better than what emerged from the evaluation of the written examination.

The final mark is calculated as a weighted average of the marks obtained in the sections of the three modules. The marks for the individual modules are calculated as follows:

MODULES OF GENERAL VETERINARY PATHOLOGY (32 points)
The written test consists of two sections. (A) Four multiple-choice questions and/or unequivocal open-ended answers on general etiology or on pathogenesis of cell damage (question 1), on adaptive mechanisms, intra- and extracellular degeneration (question 2), on inflammation (question 3) and on tumors (question 4) in this part 2 points will be assigned for each correct answer; 1 point for each partially correct answer; 0 points for each incorrect or not given answer. The maximum total score for this section of the exam is therefore 8 points (B) Four questions requiring 3 short open-ended answers on general etiology or on pathogenesis of cell damage (question 1), on adaptive mechanisms, intra- and extracellular degeneration (question 2), on inflammation (question 3) and on tumors (question 4) in this part 2 points will be assigned for each correct answer; 1 point for each partially correct answer; 0 points for each incorrect or not given answer. The maximum total score for this section of the exam is therefore 24 points

MODULE OF PATHOPHYSIOLOGY AND IMMUNOPATHOLOGY (32 points)
The written test consists of two sections. (A) Four multiple-choice questions and/or unequivocal open-ended answers on diseases of blood or plasma proteins (question 1), endocrine diseases (question 2), on circulatory diseases, organ function tests or fever, (question 3) and on immunopathology (question 4) in this part 2 points will be assigned for each correct answer; 1 point for each partially correct answer; 0 points for each incorrect or not given answer. The maximum total score for this section of the exam is therefore 8 points (B) Four questions requiring 3 short open-ended answers on diseases of blood or plasma proteins (question 1), endocrine diseases (question 2), on circulatory diseases, organ function tests or fever, (question 3) and on immunopathology (question 4) in this part 2 points will be assigned for each correct answer; 1 point for each partially correct answer; 0 points for each incorrect or not given answer. The maximum total score for this section of the exam is therefore 24 points


MODULE OF CLINICAL BIOCHEMISTRY (32 points)
The written test consists of three sections. (A) Sixteen multiple-choice and/or univocal open-answer questions in which the student must demonstrate knowledge and ability to understand the theoretical principles and practical aspects of the main biochemistry and molecular biology analysis methods used in veterinary diagnostic laboratories (16 points): 0.75 points for each correct answer; 0.4 points for each partially correct answer; 0.0 points for each incorrect answer. (B) Two open-ended questions in which the student must demonstrate knowledge of the theoretical principles and practical aspects of the main methods of biochemistry and molecular biology analysis used in veterinary diagnostic laboratories. The ability to convert an instrumental result into a biochemical diagnostic parameter will also be assessed (14 points): 10 points for the first question (general) and 4 points for the second question (specific). (C) Five calculation exercises on transformations between concentration units, preparation of solutions, calculation of enzyme activity, simple questions on the transformation of the instrumental reading of an analysis into the analyte concentration (6 points): 1.2 points for each correct answer, 0.6 points for each partially correct answer, 0.0 points for each incorrect answer.
General Veterinary pathology
Course syllabus
- Introduction (introductory concepts, i.e. homeostasis and disease) 1 hour
- General etiology (Radiations, burns; chemical causes of diseases; infectious agents; genetic-hereditary diseases) 4 hours
- Cell and tissue death (Necrosis Apoptosis) 2 hours
- Adaptive mechanisms (Hypertrophy, Hypotrophy, Metaplasia) 2 hours
- Cellular degeneration (Cell swelling, Hydropic degeneration, Vacuolar degeneration, Mucous degeneration, Fatty degeneration, Cholesterinosis - atheroma) 4 hours
- Extracellular degeneration (Jaline, fibrinoid, mucous, amyloid) 3 hours
- Acute inflammation (Inflammatory vascular changes, Cell activation, diapedesis and phagocytosis, chemical mediators, exudates, the acute phase reaction) 6 hours
- Chronic inflammation (interstitial inflammation, granuloma) 2 hours
- Tissue repair (Mechanisms of tissue repair) 2 hours
- Tumours (Terminology, neoplastic atypia, Oncogenesis and molecular oncogenesis phases of neoplastic development, metastases, cachexia and paraneoplastic syndromes, immunity against tumors) 6 hours
Teaching methods
Lectures (24 hours) with projected slides containing text, diagrams or images and videos. During lectures specific aspects will be exploited through interactive discussions and at the end of any group of topics a web based self evaluation test will be run
Teaching Resources
Slides or "on-line" version : http://ariel.ctu.unimi.it
McGAVIN e ZACHARY - Patologia Generale Veterinaria - Elsevier Masson 2008
PONTIERI - Patologia Generale -1 e 2 (2 per fisiopatologia) - Piccin. Ultima edizione
ROBBINS - Pathologic basis of the disease - 6° (2000)/7° (2004) ed. Saunders

Listed below the EAEVE day one competences (DOCs) provided during the module: 1.4, 1.13, 1.24, 2.3, 2.5.
Pathophysiology and immunopathology
Course syllabus
LECTURES
- Pathophysiology of blood cells, fluids and proteins (Volemia, anemia, polycythemia, Leucopaenia, leucocytosis, Thromboctytopenia, changes of plasma protein) 5 hours
- Pathophysiology of the endocrine system (Hyper- and hypofunction of hypophysis, thyroid, adrenal glands, including stress, diabetes) 5 hours
- Pathophysiology of metabolism (Bovine ketosis, neonatal hypoglycemia of swine, milk fever) 4 hours
- Disturbances of the circulatory system (Hyperemia, Edema, Ischemia, Infarct, Thrombosis, Embolizaton, Shock) 2 hours
- Pathophysiology of thermoregulation (febrile and non febrile hyperthermia) 1 hour
Jaundice. 1 hour
- Organ function tests (Principles of interpretation, tests for liver functions) 1 hour
Immunopathology (Hypersensitivity reactions (Type I, II, III, IV), Autoimmune diseases, Immunodeficiencies 5 hours

PRACTICALS
- Laboratory methods (seminar in the classroom to the whole studentship) 2 hours
Preanalytical and analytical errors (seminar in the classroom to the whole studentship) 2 hours
- Handling and analysis of blood (max 20 students per group in the laboratory) Cell counts, execution of blood smears, Centrifugation and biochemical analysis using automated methods 2 hours
- Reading of blood smears (max 30 student per group with individual microscopes) Approach to the blood smear at the microscope, execution of the differential leukocyte count, identification of the main pathological changes of RBC, WBC, and platelets 8 hours
Principles of test interpretation and self-evaluation test 2 hours
Teaching methods
Lectures (24 hours) with projected slides containing text, diagrams or images and videos. During lectures specific aspects will be exploited through interactive discussions and at the end of any group of topics a web based self evaluation test will be run
Practicals (16 hours) will be run as specified at the end of the programme in teaching labs (microscopy and biological lab) within the teaching buildings
Teaching Resources
- Slides or "on-line" version : http://ariel.ctu.unimi.it
- PONTIERI - Patologia Generale -1 e 2 (2 per fisiopatologia) - Piccin. Ultima edizione
- TIZARD - Veterinary Immunolgy - An Introduction, 6th ed. Saunders

For specific interests (hematology and andocrine diseases)
- WEISS e WARDROP- Schalm's Veterinary Hematology - 6° ed. Lippincott, Williams & Wilkins
KANEKO - Clinical Biochemistry of domestic animals - 6° ed.
- STOCKHAM & SCOTT - Fundamentals of Veterinary Clinical Pathology - 2° ed.
- PALTRINIERI GIORDANO, BERTAZZOLO - Patologia Clinica del Cane e del gatto. Approccio pratico alla diagnostica di laboratorio

Listed below the EAEVE day one competences (DOCs) provided during the module: 1.3, 1.4, 1.5, 1.11, 1.15, 1.21, 1.22, 1.28, 2.1, 2.3, 2.5, 2.11
Clinical biochemistry
Course syllabus
LECTURES (24 h)
- Ways of expressing the quantity in clinical biochemistry; International System of Units and conversion of units of measure (w/v, molarity, ppm, ppb, percent). (2h)
- Methods based on radiant energy: basic principles of light transmission, absorption, refraction, diffraction, reflection and production. (1h)
- From the instrument reading to the concentration of the analyte. (2h)
- Clinical chemistry methods. (1h)
- Determination of metabolites by enzymatic methods. (1h)
- Clinical enzymology: enzymatic activity and kinetics; determination of enzymes in plasma; continuous assays, discontinuous assays, factors to control in assays. (2h)
- Immunochemical techniques for antibody and antigen quantification; direct and indirect (labelled) immunological assays; competitive and sandwich assays; lateral-flow test. (2h)
- Electrophoresis: net charge of biomolecules; net charge electrophoresis; SDS-electrophoresis; Western blot; the electropherogram and its interpretation. (2h)
- Electrochemical techniques: potentiometric measures with ion selective electrodes; amperometric techniques. (1h)
- Atomic spectroscopy: atomic absorption and emission techniques. (1h)
- Molecular biology methods: PCR, analysis of PCR products, sequencing. (2h)
- Source of error of laboratory's test result: pre-analytical variability, analytical variability: precision, accuracy, sensitivity, specificity, cross-reactivity, species-specificity. Quality control (4h)
- The use of clinical laboratory test in diagnostic: biological variability, reference values; pathognomonic and non-pathognomonic tests; diagnostic sensitivity and specificity; prevalence; positive and negative predictive values. (3h)

PRACTICALS (16h)
- Exercises on conversion units of measure (w/v, molarity, percentage, ppm, ppb), and on preparing solution and dilution. (12h, classroom)
- Laboratory mathematics: processing of raw data from quantitative assays of biomarkers, graphical representation, and critical analysis of test results. Use of Excel spreadsheets to calculate the concentration of the analytes from instrument raw data obtained from: enzymatic creatinine assay, sandwich and competitive ELISAs, net charge-based electrophoresis (electropherogram) (2h, computer room).
- Use of NCBI software and databases for the species identification of nucleotide sequences obtained by PCR/sequencing and design of species-specific primers (2h, computer room).
Teaching methods
- Lectures (24 hours). Classroom lectures with slide projections in which principles and applications of the main biochemical and clinical molecular biology methodologies used in the veterinary biochemical diagnostic laboratory are discussed.

- Practical training (16 hours). These are held in the classroom for all students and in the computer room with students divided into groups. They are aimed at carrying out laboratory mathematics exercises and calculations for the processing of numerical instrumental results into biochemical diagnostic parameter concentrations.
Teaching Resources
- Power point handouts and lecture notes on ARIEL - Study books
- Biochimica clinica, 2° edizione (2000), Luigi Spandrio, Edizioni Sorbona. Prima Parte (Biochimica Clinica Generale).

For further study:
- Metodologia biochimica - Keith Wilson e John Walker, 5° edizione - Eds Italiana Pilone e Pollegioni, Raffaello Cortina Editore (2006). Clinical biochemistry of domestic animals, 6° edizione (2008), Kaneko JJ, Academic press.
- Clinical biochemistry of domestic animals, 6° edizione (2008), Kaneko JJ, Academic press.
Clinical biochemistry
BIO/12 - CLINICAL BIOCHEMISTRY AND MOLECULAR BIOLOGY - University credits: 4
Practicals: 16 hours
Lessons: 24 hours
Professor: Borromeo Vitaliano
Shifts:
1- turno per un gruppo di studenti
Professor: Borromeo Vitaliano
2- turno per un gruppo di studenti
Professor: Borromeo Vitaliano
Turno
Professor: Borromeo Vitaliano
Turno unico per tutti gli studenti
Professor: Borromeo Vitaliano
General Veterinary pathology
VET/03 - VETERINARY PATHOLOGY - University credits: 4
Lessons: 32 hours
Shifts:
Turno
Professor: Paltrinieri Saverio
Pathophysiology and immunopathology
VET/03 - VETERINARY PATHOLOGY - University credits: 4
Practicals: 16 hours
Lessons: 24 hours
Shifts:
1- 4 turni da 2 ore per gruppi di studenti
Professor: Paltrinieri Saverio
2- 3 turni da 8 ore per un gruppo di studenti
Professor: Paltrinieri Saverio
TURNO UNICO PER TUTTI GLI STUDENTI
Professor: Paltrinieri Saverio
Turno
Professor: Paltrinieri Saverio