Techniques for Advanced Biomedical Research
A.Y. 2024/2025
Learning objectives
Aim of the course is to provide basic and applied knowledge of the most updated techniques used in biomedical research. The course will be characterized by a multidisciplinary approach in order to provide a range of up-to-date and timely information on new technological developments, useful to professional application and research activity.
Expected learning outcomes
In the last years the biomedical research has faced an impressive advancement of the methodological tools. This course intends to expose the students to a large number of these techniques so to build a solid knowledge of the most novel methodological information. The students will benefit from this course since they will acquire the competence to identify the most appropriate and powerful techniques to use for their research purposes.
Lesson period: First semester
Assessment methods: Esame
Assessment result: voto verbalizzato in trentesimi
Single course
This course can be attended as a single course.
Course syllabus and organization
Single session
Responsible
Lesson period
First semester
Course syllabus
The course is characterized by thematic issues discussed in the classroom or, when necessary, directly in the laboratories. The following is a list of the techniques that will be presented:
-Cloning techniques, genetic manipulation, and heterologous expression;
-CRISP-CAS9;
-Stem cells: an experimental model to unravel pathophysiological mechanisms as well as element for tissue regeneration;
-Methods used in the study of the processes associated with angiogenesis;
-Electrophysiological techniques (patch-clamp) on single cells;
-Telemetric measurements of cardiac activity (ECG) in freely-moving animals;
-Complementary experimental methodologies to study 2D cardiomyocytes and/or neuronal cultures;
-Analytical techniques for proteomic studies;
-Analytical techniques for metabolomic studies;
-Tissue micro-Arrays;
-Capillary electrophoresis:
-Nanotechnologies;
-Techniques for advanced functional microscopy, confocal microscopy, electronic microscopy;
- Fluorescence Activated Cell Sorting (FACS);
-Next Generation Sequencing and identification of mutations associated with pathologies.
-Cloning techniques, genetic manipulation, and heterologous expression;
-CRISP-CAS9;
-Stem cells: an experimental model to unravel pathophysiological mechanisms as well as element for tissue regeneration;
-Methods used in the study of the processes associated with angiogenesis;
-Electrophysiological techniques (patch-clamp) on single cells;
-Telemetric measurements of cardiac activity (ECG) in freely-moving animals;
-Complementary experimental methodologies to study 2D cardiomyocytes and/or neuronal cultures;
-Analytical techniques for proteomic studies;
-Analytical techniques for metabolomic studies;
-Tissue micro-Arrays;
-Capillary electrophoresis:
-Nanotechnologies;
-Techniques for advanced functional microscopy, confocal microscopy, electronic microscopy;
- Fluorescence Activated Cell Sorting (FACS);
-Next Generation Sequencing and identification of mutations associated with pathologies.
Prerequisites for admission
Basic concepts of physics, physiology, genetics, and molecular biology.
Teaching methods
Traditional lectures are supported by interactive material. When possible the lectures will take place in the labs where instruments are placed. The teachings will benefit from the participation of highly qualified experts of the specific methodologies.
Teaching Resources
Teaching material will be made available through the Ariel website or specifically suggested during the lectures.
Assessment methods and Criteria
The exam consists of a written test (duration of 60 minutes) organized into 2 assays on topics presented in class. An additional oral discussion is required when the written test is not recognized as sufficient to determine the level of knowledge reached by the student. Final grades will be posted on the Ariel platform.
BIO/09 - PHYSIOLOGY - University credits: 6
Lessons (seminars): 48 hours
Professors:
Baruscotti Mirko, Bucchi Annalisa
Professor(s)
Reception:
Wednesday from 11:00AM to 12:00AM by appointment
Via Celoria 26 - VI° Piano Torre A Edifici Biologici