Principles of Quantitative Pharmaceutical Analysis and Laboratory for Quantitative Analysis

A.Y. 2024/2025

Max ECTS

Overall hours

SSD

CHIM/08

Language

Italian

Included in the following degree programmes

Pharmacy (Classe LM-13)-Enrolled from 2009/10 Until 2021/22 Academic Year

Pharmacy (Classe LM-13)-Enrolled in 2022/23 Academic Year

Learning objectives

Two didactic units composes the course: lectures (Principles of Quantitative Pharmaceutical Analysis) and individual laboratory training (Laboratory for Quantitative Analysis).
The objectives of the lectures consist in providing the knowledge needed to apply the analytical methods, already partially learned in the preparatory courses (General and Inorganic Chemistry, and Stoichiometry, Analytical Chemistry and Inorganic Analysis of Substances having Pharmaceutical Interest and Qualitative Analysis Laboratory), described in the current European Pharmacopoeia for the quantitative analysis of substances for pharmaceutical use.
Concerning the experimental practices, the students will acquire the skills needed for the dosage of pharmaceutical substances reported in the Italian and European Pharmacopoeias.

Expected learning outcomes

By the lectures, the students will have improved further their knowledge concerning the theoretical and practical aspects related to the quantitative analysis, which they will have applied during the individual experimental practices.
The laboratory experiences (mandatory) will have provided to the students the skills to perform the quantitative analysis of pharmaceutical compounds, to complete their knowledge (by means also of other courses as Assays and Methods of Analysis of the Pharmacopoeias and Laboratory of Drug Identification).

Lesson period: Second semester

Lessons timetable

Assessment methods: Esame
Assessment result: voto verbalizzato in trentesimi

Exams calendar

Single course

This course can be attended as a single course.

Take a single course

Course syllabus and organization

Linea AL

Responsible

Gelain Arianna

Lesson period

Second semester

Syllabus
Course structure

Course syllabus

Programme of the lectures:
Introduction to the principles of medicinal chemical- quantitative analysis.
The current European Pharmacopea as a reference text for quantitative analyses of active ingredients, excipients, plant extracts and nutrients.
Statistical processing and evaluation of analytical data.
Development and validation of an analytical method.
Gravimetric analysis.
Volumetric analysis: definition of direct titrations, back- and indirect-titrations; acid-base titrations; precipitation-forming titration, oxidation-reduction titrations; complex-formation titrations; potentiometric titrations - non-aqueous titrations.
Analytical techniques described in the current European Pharmacopea applied to quantitative analysis.
Introduction to analytical techniques; potentiometry; elements of voltammetry and amperometry; optical methods: UV-Visible spectroscopy, fluorescence and phosphorescence; chromatography techniques: high-performance liquid chromatography, gas-chromatography and headspace analysis.

Programme of the laboratory exercises:
- Description of the analytical laboratory hardware (e.g. glassware, instruments, etc.);
- Complexation titrations
- Oxidation-reduction titration (permanganometry, iodimetry, Iodometry etc.)
- Acid- base titrations
- Precipitation titrations.

Prerequisites for admission

The course takes place in the second semester of the second year. The students are expected to have Analytical Chemistry, Analysis of inorganic substances for pharmaceutical use and Laboratory of qualitative analysis and Organic Chemistry knowledges.

Teaching methods

The course is structured in lectures and laboratory exercises (mandatory).
All information about the program and educational materials (slide) are available on the course website on myAriel platform.

Teaching Resources

Recommended books:
Chimica analitica quantitativa - D. C. Harris - Zanichelli
Chimica analitica - Skoog, West, Holler - EdiSES
Chimica analitica e analisi quantitativa - D. S. Hage, J. D. Carr - Piccin
Principi di Analisi Farmaceutica - Cavrini V. Andrisano V. - Esculapio 2017
Analisi strumentale - Bauer, Christian, O'Reilly - Piccin
Analisi di preparazioni farmaceutiche -Giulio Cesare Porretta - CISU
Vogel's Textbook of quantitative Chemical analysis - Longman Eds
European Pharmacopoeia Online - EDQM website

Assessment methods and Criteria

The examination consists of a written test (120 minutes duration) based on problems related to the stechiometry and the chemical equilibrium of the various titration reactions, by critical evaluation of the analytical data, and questions concerning the theory of quantitative analysis and the application of the various analytical methods. The overall assessment is scored in thirtieth and it corresponds to the sum of the scores obtained in the written test (maximum score 24/30) and in the laboratory exercises (maximun score 6/30). The final score of the examination is communicated during the return and correction of the test. In the course of the lectures, ongoing exercises are proposed in preparation for the examination.

Laboratory for quantitative analysis

CHIM/08 - PHARMACEUTICAL CHEMISTRY - University credits: 2

Single bench laboratory practical: 32 hours

Professor: Straniero Valentina

Shifts:

Turno 1

Professor: Straniero Valentina

Principles of quantitative pharmaceutical analysis

CHIM/08 - PHARMACEUTICAL CHEMISTRY - University credits: 6

Lessons: 48 hours

Professor: Gelain Arianna

Linea MZ

Responsible

Fumagalli Laura

Lesson period

Second semester

Syllabus
(active tab)
Course structure

Course syllabus

Principles of Quantitative Pharmaceutical Analysis (Lessons)
Introduction to the principles of medicinal chemical- quantitative analysis.
The current European pharmacopea as a reference text for quantitative analyses of active ingredients, excipients, plant extracts and nutrients.
Statistical processing and evaluation of analytical data.
Development and validation of an analytical method.
Gravimetric analysis.
Volumetric analysis: Definition of direct titrations, back- and indirect-titrations; acid-base titrations; precipitation-forming titration, oxidation-reduction titrations; complex-formation titrations; potentiometric titrations - nonaqueous titrations.
Analytical techniques described in the current European Pharmacopea applied to quantitative analysis.
Introduction to analytical techniques; optical methods; UV-Visible spectroscopy, fluorescence and phosphorescence; chromatography techniques; high-performance liquid chromatography; gas-chromatography and Headspace analysis.
Program of the practical laboratory experiences:
- Illustration of the analytical laboratory hardware (e.g. glassware, instruments, etc.);
- complexation titrations;
- oxidation-reduction titration (permanganometry, iodimetry, Iodometry .
- acid- base titrations;
- precipitation titrations.

Prerequisites for admission

The student must have a good knowledge of General and Analytical Chemistry and, in particular, of the chemical solution equilibriums.

Teaching methods

Lectures and laboratory experiences (mandatory).
All the info about the program and books/slides are available on myAriel platform.

Teaching Resources

All info about the program, books/slides are available on myARIEL website.
Recommended Books:
Chimica analitica quantitativa - D. C. Harris - Zanichelli
Chimica analitica - Skoog, West, Holler - EdiSES
Chimica analitica e analisi quantitativa - D. S. Hage, J. D. Carr - Piccin
Principi di Analisi Farmaceutica - Cavrini V. Andrisano V. - Esculapio 2017
Analisi strumentale - Bauer, Christian, O'Reilly - Piccin
Analisi di preparazioni farmaceutiche -Giulio Cesare Porretta - CISU
Vogel's Textbook of quantitative Chemical analysis - Longman Eds
European Pharmacopoeia Online - EDQM website

Assessment methods and Criteria

The verification of the learning is carried out by a written test (90 minutes).
The examination consists of a written test based on the resolution of problems related to the stechiometry and the chemical equilibrium of the various titration's reactions and open-ended questions concerning the theory of quantitative analysis covered during the course. The marks obtained is scored in thirtieth and results from the sum of the scores obtained in the written test (maximum score 24/30) and in the laboratory exercises (maximun score 6/30). During the course ongoing exercises are proposed in preparation for the exam.

Laboratory for quantitative analysis

CHIM/08 - PHARMACEUTICAL CHEMISTRY - University credits: 2

Single bench laboratory practical: 32 hours

Professor: Fumagalli Laura

Shifts:

Turno 2

Professor: Fumagalli Laura

Principles of quantitative pharmaceutical analysis

CHIM/08 - PHARMACEUTICAL CHEMISTRY - University credits: 6

Lessons: 48 hours

Professor: Fumagalli Laura

Educational website(s)

Principi di Analisi farmaceutica quantitativa e Lab. di Analisi quantitativa Linea A-L (a.a. 2024/25)
Principi di Analisi farmaceutica quantitativa e Laboratorio di analisi quantitativa Linea M-Z (a.a. 2024/25)

Professor(s)

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