Plant Microbiome-Based Strategies for Agri-Environmental Biotechnologies

A.Y. 2024/2025
4
Max ECTS
36
Overall hours
SSD
AGR/16
Language
English
Learning objectives
The course aims to provide students the tools to exploit the knowledge on the beneficial interaction between plants and the associated microbiomes to develop biotechnological approaches to boost plant fitness and resilience, crop productivity in different ecosystems (agricultural soils, extreme environment) and under different conditions (biotic/abiotic stresses) and increase sustainability in agricultural practice.
Expected learning outcomes
At the end of the course the student will be able to: i) analyse the plant microbiome in terms of definition, microbial components and plant microbial niches (rhizosphere, endosphere, phyllosphere, seed, shoot); ii) illustrate the main ecological factors tuning the assembly and remodelling of the plant associated microbiomes in soil; iii) illustrate the main methodologies (metabolomics, metagenomics, genomics, transcriptomics, culturomics) to decipher the plant-microbiome interactions under control conditions and under biotic/abiotic stress; iv) analyse synthetic communities (SynCom) as a tool to engineer the plant microbiome to improve the plant fitness in agricultural ecosystems; v) discuss the composition, role and application of biostimulants to support sustainable practice in agriculture and for land reclamation; vi) analyse the diversity, structure, role and transmission of the seed microbiota in the light of agricultural management practice; vii) discuss the research challenges to be faced to improve plant health and productivity through the associated microbiome manipulation.
Single course

This course cannot be attended as a single course. Please check our list of single courses to find the ones available for enrolment.

Course syllabus and organization

Single session

Responsible
Lesson period
Second semester
Course syllabus
- The holobiont concept, plant and soil ecological niches
- Methodology to investigate plant-microbiome interactions (Next Generation Sequencing techniques, culturomics, microscopy, omics-techniques like transcriptomics, metabolomics, metagenomics, proteomics)
- Main plant microbiome components: bacteria, fungi, archea, protists and virus
- Factors influencing the structure and communication in plant-microbiome interactions
- Mechanisms of microbiome-driven plant growth promotion
- The plant microbiome for improving plant resistance to abiotic stresses
- Microbiome engineering for sustainable agriculture
- Microbial consortia and synthetic communities
- Biofertilizers, biostimulators and biopesticides
- The seed microbiome
- The phyllosphere microbiome
- Weed management through manipulation of the plant microbiome
- Agriculture in the Anthropocene
- The microbiome for land reclamation
- Case studies
Prerequisites for admission
General knowledge on environmental microbiology, molecular biology and biotechnology are essential to attend this course. You can contact the teacher to obtain indications about texts and bibliographic references that can be studied to fill eventual knowledge gaps.
Teaching methods
The course consists in 4 CFU, including lectures (3.5 CFU) and laboratory exercise (0.5 CFU).
Teaching Resources
Powerpoint/pdf presentation of each lecture will be provided to the students, including additional material like scientific papers, reviews and web links. The material will be uploaded on myAriel during the course.
Assessment methods and Criteria
Course final examination foresees a written exam consisting in 3 open questions on specific topics described during the course. The students will have 1.5 hours to reply to the questions.
Specific procedures for students with disabilities or specific learning disabilities (DSA) will be applied. Here the complete information:
https://www.unimi.it/en/study/student-services/services-students-specific-learning-disabilities-sld
https://www.unimi.it/en/study/student-services/services-students-disabilities
In case you need specific procedures, please inform the teacher by mail at least 10 days before the exam, including in copy to the communication also: [email protected] and [email protected].
AGR/16 - AGRICULTURAL MICROBIOLOGY - University credits: 4
Practicals: 8 hours
Lessons: 28 hours
Professor: Rolli Eleonora
Shifts:
Turno
Professor: Rolli Eleonora
Professor(s)