Diagnostic Techniques in Microbiology, Virology, Micology and Parasitology
A.Y. 2024/2025
Learning objectives
The aim of the course is to provide:
1. the theoretical-practical knowledge of the most common laboratory diagnostic techniques applied in bacteriology, virology, mycology and parasitology in order to be able to carry out a correct diagnostic process in various pathological materials.
2. An overview of the organization and safety criteria of the clinical microbiology laboratory
1. the theoretical-practical knowledge of the most common laboratory diagnostic techniques applied in bacteriology, virology, mycology and parasitology in order to be able to carry out a correct diagnostic process in various pathological materials.
2. An overview of the organization and safety criteria of the clinical microbiology laboratory
Expected learning outcomes
Student will be able to apply the most relevant analytical procedures in clinical microbiology. He will also learn the most important diagnostic algorithm in the field of clinical microbiology.
At the end of the course the student will be able to perform the most basic microbiological tests for a correct diagnosis. She/he will apply techniques including the basic gram-stain till the most relevant and difficult diagnostic assays.
At the end of the course the student will be able to perform the most basic microbiological tests for a correct diagnosis. She/he will apply techniques including the basic gram-stain till the most relevant and difficult diagnostic assays.
Lesson period: First semester
Assessment methods: Esame
Assessment result: voto verbalizzato in trentesimi
Single course
This course can be attended as a single course.
Course syllabus and organization
Single session
Responsible
Prerequisites for admission
The student must have knowledge in the field of microbiology and how to categorize and classify microorganisms on the basis of their related diseases. He/she will therefore need to know the main viruses, bacteria and fungi of medical interest, as well as the technical bases for their phenotypic and genotypic identifications.
Assessment methods and Criteria
The exam will in a written form by including all the topics listed in the program. In particular, each student will receive a written test with open questions and multiple choice cross questions If required, in particular case, there is the possibility to do oral examination.
The evaluation is a grade expressed out of thirty.
The evaluation is a grade expressed out of thirty.
Microbiology and clinical microbiology
Course syllabus
BACTERIOLOGICAL EXAMINATION AND DIFFERENTIAL STAININGS METHODS
PREVENTION OF BIOLOGICAL RISK:
· Review of the use of individual protection devices, air flow cabinets management and immunological surveillance.
PRINCIPLES OF VIROLOGICAL DIAGNOSYS:
· CULTURAL EXAMINATION;
· tissue and cell cultures;
· Direct and indirect immunofluorescence tests for identification of specific virus antigens
· Molecular Biology Techniques
SEROLOGICAL DIAGNOSIS AND CONFIRMATORY TESTS.
URINARY TRACT INFECTIONS:
· MAIN PATHOGENES;
· Laboratory diagnosis.
INFECTIONS OF THE GASTROINTESTINAL TRACT:
· MAIN PATHOGENES;
· Laboratory diagnosis.
MICROBACTERIA AND M.TUBERCULOSIS:
· Diagnosis of infection (culture test, microscopic examination and PCR).
RESPIRATORY TRACT INFECTIONS:
MAIN PATHOGENES;
· Laboratory diagnosis.
INFECTIONS OF THE CENTRAL NERVOUS SYSTEM: - main pathogens;
· Laboratory diagnosis.
GENITAL TRACT INFECTIONS:
· MAIN PATHOGENES;
· Laboratory diagnosis.
DIAGNOSTICS OF NON-VIRAL SEXUALLY TRANSMITTED MICROORGANISMS:
MYCOPLASMA, CHALAMYDIA TRACHOMATIS, NEISSERIA GONORRHOEAE:
· Laboratory diagnosis
DIAGNOSTIC PROTOCOLS FOR INFECTIONS RELATED TO VASCULAR CATHETER.
VIRUS OF HEPATITES A, B, C AND D AND VIRUS HIV:
· Main characteristics and laboratory diagnosis
PREVENTION OF BIOLOGICAL RISK:
· Review of the use of individual protection devices, air flow cabinets management and immunological surveillance.
PRINCIPLES OF VIROLOGICAL DIAGNOSYS:
· CULTURAL EXAMINATION;
· tissue and cell cultures;
· Direct and indirect immunofluorescence tests for identification of specific virus antigens
· Molecular Biology Techniques
SEROLOGICAL DIAGNOSIS AND CONFIRMATORY TESTS.
URINARY TRACT INFECTIONS:
· MAIN PATHOGENES;
· Laboratory diagnosis.
INFECTIONS OF THE GASTROINTESTINAL TRACT:
· MAIN PATHOGENES;
· Laboratory diagnosis.
MICROBACTERIA AND M.TUBERCULOSIS:
· Diagnosis of infection (culture test, microscopic examination and PCR).
RESPIRATORY TRACT INFECTIONS:
MAIN PATHOGENES;
· Laboratory diagnosis.
INFECTIONS OF THE CENTRAL NERVOUS SYSTEM: - main pathogens;
· Laboratory diagnosis.
GENITAL TRACT INFECTIONS:
· MAIN PATHOGENES;
· Laboratory diagnosis.
DIAGNOSTICS OF NON-VIRAL SEXUALLY TRANSMITTED MICROORGANISMS:
MYCOPLASMA, CHALAMYDIA TRACHOMATIS, NEISSERIA GONORRHOEAE:
· Laboratory diagnosis
DIAGNOSTIC PROTOCOLS FOR INFECTIONS RELATED TO VASCULAR CATHETER.
VIRUS OF HEPATITES A, B, C AND D AND VIRUS HIV:
· Main characteristics and laboratory diagnosis
Teaching methods
Frontal lessons assisted by visual tools.
Teaching Resources
Michele La Placa: Principi di Microbiologia Medica, Edises s.r.l., Napoli
Patrick R. Murray, Ken S. Rosenthal, Michael A. Pfaller: Microbiologia Medica, Edra Masson, Milano
Patrick R. Murray, Ken S. Rosenthal, Michael A. Pfaller: Microbiologia Medica, Edra Masson, Milano
Medical and biotechnology laboratory techniques
Course syllabus
Main protozoa responsible for human infections:
· Giardia intestinalis
· Trichomonas vaginalis
· Leishmania spp.
· Tripanosoma spp.
· Entamoeba histolytica
· Naegleria fowleri
· Plasmodium spp.
· Toxoplasma gondii
Type and treatment of microbiological samples:
. Bronchial aspirate and escreate
. Faecal samples
. Urine
. Blood culture and cerebral spinal fluid
· Oro-pharyngeal samples
. Vaginal samples
Classroom exercises
· Giardia intestinalis
· Trichomonas vaginalis
· Leishmania spp.
· Tripanosoma spp.
· Entamoeba histolytica
· Naegleria fowleri
· Plasmodium spp.
· Toxoplasma gondii
Type and treatment of microbiological samples:
. Bronchial aspirate and escreate
. Faecal samples
. Urine
. Blood culture and cerebral spinal fluid
· Oro-pharyngeal samples
. Vaginal samples
Classroom exercises
Teaching methods
Frontal lessons assisted by visual tools.
Classroom exercises
Classroom exercises
Teaching Resources
Michele La Placa: Principi di Microbiologia Medica, Edises s.r.l., Napoli; - G. Antonelli, G. Pozzi, M. Clementi, G.M. Rossolini: Principi di Microbiologia Medica IV ed., CEA; Eudes Lanciotti: Microbiologia clinica, Zanichelli
Medical and biotechnology laboratory techniques
MED/46 - BIOTECHNOLOGY AND METHODS IN LABORATORY MEDICINE - University credits: 2
Lessons: 20 hours
Professor:
Parapini Silvia
Shifts:
Turno
Professor:
Parapini Silvia
Microbiology and clinical microbiology
MED/07 - MICROBIOLOGY AND CLINICAL MICROBIOLOGY - University credits: 2
Lessons: 20 hours
Professor:
Drago Lorenzo
Shifts:
Turno
Professor:
Drago LorenzoProfessor(s)
Reception:
11.00 - 12.00 on Monday by appointment
Direzione Laboratorio Clinico Generale con Aree Specialistiche. IRCCS Multimedica, Via Fantoli 15/16, Milano (Italy). Tel. 02.5540 6642 - Laboratorio di Microbiologia Clinica, Via Mangiagalli, 31 - Milano