Biomolecular Methodologies
A.Y. 2024/2025
Learning objectives
The course aims to provide students with the methodological foundations of biochemical approaches for the analysis and characterization of food material in terms of quality and safety.
Expected learning outcomes
At the end of the course the students will acquire the ability to interpret and critically analyze the results obtained through biochemical methodologies for the characterization of the food material. Students will also be able to identify the most suitable approaches to the definition of molecular quality indexes of a food.
Lesson period: Second semester
Assessment methods: Esame
Assessment result: voto verbalizzato in trentesimi
Single course
This course can be attended as a single course.
Course syllabus and organization
Single session
Responsible
Lesson period
Second semester
Course syllabus
Formal classes; fundamentals of the principal biochemical approaches for separation, identification and quantification of biological macromolecules, and their application in defining the quality of ingredients and/or in foods: - chromatography: gel filtration, ion exchange, hydrophobic and reverse phase chromatography - electrophoresis: SDS-PAGE, IEF, 2D electrophoresis - application of mass spectrometry to food issues - immunochemical and immunoenzymatic techniques: dot-blotting, Western blotting, and ELISA Spectroscopic approaches to study the structural modifications of macromolecules and their relevance to food quality and consumerism. Application of molecular biology approaches to food safety issues. Presentation and critical analysis of "case studies".
Pactical laboratories on the following topics: spectroscopic approaches; electrophoresis; chromatographic techniques; immunoenzymatic techniques
Pactical laboratories on the following topics: spectroscopic approaches; electrophoresis; chromatographic techniques; immunoenzymatic techniques
Prerequisites for admission
Fundamentals of structural and physico-chemical features of biological systems. Basic knowledge of biological macromolecules.
Teaching methods
Formal classes; fundamentals of the principal biochemical approaches for separation, identification and quantification of biological macromolecules, and their application in defining the quality of ingredients and/or in foods: - chromatography: gel filtration, ion exchange, hydrophobic and reverse phase chromatography - electrophoresis: SDS-PAGE, IEF, 2D electrophoresis - application of mass spectrometry to food issues - immunochemical and immunoenzymatic techniques: dot-blotting, Western blotting, and ELISA Spectroscopic approaches to study the structural modifications of macromolecules and their relevance to food quality and consumerism. Application of molecular biology approaches to food safety issues. Presentation and critical analysis of "case studies".
Pactical laboratories on the following topics: spectroscopic approaches; electrophoresis; chromatographic techniques; immunoenzymatic techniques.
Pactical laboratories on the following topics: spectroscopic approaches; electrophoresis; chromatographic techniques; immunoenzymatic techniques.
Teaching Resources
Original slides from the ARIEL website
Reference textbooks:
Campbell/Farrel, Biochimica, EdiSES;
M Maccarrone Metodologie Biochimiche e biomolecolari, Zanichelli
MC Bonaccorsi di Patti R Contestabile ML Di Salvo Metodologie Biochimiche, Zanichelli
De Marco/Cini, Principi di Metodologia Biochimica, Piccin
Reference textbooks:
Campbell/Farrel, Biochimica, EdiSES;
M Maccarrone Metodologie Biochimiche e biomolecolari, Zanichelli
MC Bonaccorsi di Patti R Contestabile ML Di Salvo Metodologie Biochimiche, Zanichelli
De Marco/Cini, Principi di Metodologia Biochimica, Piccin
Assessment methods and Criteria
Oral exams, carried out face-to-face. Preliminary registration to the exam session through the UNIMIA system is mandatory.
DSA students and students with disabilities are invited to inform the teacher by e-mail at least fifteen days before the exam date in order to define the possible individual actions. The e-mail should also send in cc to: [email protected] (for DSA students) or [email protected] (for students with disabilities).
DSA students and students with disabilities are invited to inform the teacher by e-mail at least fifteen days before the exam date in order to define the possible individual actions. The e-mail should also send in cc to: [email protected] (for DSA students) or [email protected] (for students with disabilities).
BIO/10 - BIOCHEMISTRY - University credits: 4
Laboratories: 16 hours
Lessons: 24 hours
Lessons: 24 hours
Professors:
Emide Davide, Iametti Stefania
Shifts:
Professor(s)