Biochemestry
A.Y. 2024/2025
Learning objectives
General aims of the course: (1) To introduce students to the molecular design of life, including structural and biological properties of the main constituents of organisms; (2) To describe the molecular basis of the main biological processes, with particular emphasis on the metabolic fate of the lipidic, proteic and glicidic components of food; (3) To detail molecular basis of energy production from the main food components under aerobic and anaerobic conditions; (4) To introduce students to the main molecular biology mechanisms, including DNA replication, RNA and protein synthesis; (5) To detail the structure-function relationship in proteins.
Expected learning outcomes
1. Knowledge and understanding: the student, at the end of the course, will have to demonstrate to have basic knowledge relating to animal metabolism, with special emphasis on animal productions, in order to understand the structural/energetic/reserve role of the most relevant biomolecules. Biochemical aspects linked to muscle metabolism will also be explored in depth. The student will also have to acquire knowledge relating to the structure and role of informational macromolecules (proteins and nucleic acids) as well as the main mechanisms involved in the regulation of biological processes. Theoretical-practical aspects of some basic biochemical laboratory techniques will also be presented.
2. Applying knowledge and understanding: At the end of the course, the student must be able to distinguish the main metabolic effects of the lipid, carbohydrate and protein components present in foods, with particular attention to their energy intake. This basic knowledge will be necessary to understand other aspects relating to animal welfare. Furthermore, the student must be able to understand some basic biochemical techniques useful in the analysis of biological samples and foods.
3. Making judgments: the student must demonstrate the ability to critically argue the information acquired. During the course, students are encouraged to think about practical examples and group discussions.
4. Communication: the student must demonstrate the ability to use scientifically appropriate terminology, in particular concerning the terminology referring to the structure and function of the main biological molecules, metabolic processes of the main components of foods, replication processes of DNA and the biosynthesis of RNA and proteins. The exercises and seminars will stimulate the ability to express oneself correctly and the ability to discuss scientifically with peers.
5. Lifelong learning skills: the student must demonstrate the ability to use the knowledge acquired to interpret structures of macromolecules and new metabolic pathways, searching scientific databases and critically organizing new information.
2. Applying knowledge and understanding: At the end of the course, the student must be able to distinguish the main metabolic effects of the lipid, carbohydrate and protein components present in foods, with particular attention to their energy intake. This basic knowledge will be necessary to understand other aspects relating to animal welfare. Furthermore, the student must be able to understand some basic biochemical techniques useful in the analysis of biological samples and foods.
3. Making judgments: the student must demonstrate the ability to critically argue the information acquired. During the course, students are encouraged to think about practical examples and group discussions.
4. Communication: the student must demonstrate the ability to use scientifically appropriate terminology, in particular concerning the terminology referring to the structure and function of the main biological molecules, metabolic processes of the main components of foods, replication processes of DNA and the biosynthesis of RNA and proteins. The exercises and seminars will stimulate the ability to express oneself correctly and the ability to discuss scientifically with peers.
5. Lifelong learning skills: the student must demonstrate the ability to use the knowledge acquired to interpret structures of macromolecules and new metabolic pathways, searching scientific databases and critically organizing new information.
Lesson period: Second semester
Assessment methods: Esame
Assessment result: voto verbalizzato in trentesimi
Single course
This course can be attended as a single course.
Course syllabus and organization
Single session
Responsible
Lesson period
Second semester
Course syllabus
Theoretical lectures (tot.48 hours):
Protein structural levels (2 hours)
Collagen (2 hours)
Membranes and membrane transport (2 hours)
Myoglobin and hemoglobin (2 hours)
Enzymes, mechanism and regulation of enzyme action. Zymogen (2 hours)
Molecular mechanism of digestion, absorption and transport of biomolecules (2 hours)
Metabolism and bioenergetics (2 hours)
Carbohydrates metabolism: glycolysis, pentose phosphate pathway, glycogen, gluconeogenesis, synthesis of lactose (4 hours)
TCA cycle and oxidative phosphorylation (2 hours)
Fatty acid catabolism and lipid biosynthesis. Ketone bodies. Cholesterol metabolism (4 hours)
Amino acid metabolism, ammonia metabolism and urea cycle (3 hours)
Nucleotides: purines and pyrimidines metabolism (2 hours)
Metabolic integration and intra-extra cellular regulation (2 hours)
Nucleic acids and biological function. Genetic code. DNA replication, transcription, gene expression and protein synthesis (11 hours)
Rumen biochemistry (4 hours)
Lab technologies (2 hours)
Protein structural levels (2 hours)
Collagen (2 hours)
Membranes and membrane transport (2 hours)
Myoglobin and hemoglobin (2 hours)
Enzymes, mechanism and regulation of enzyme action. Zymogen (2 hours)
Molecular mechanism of digestion, absorption and transport of biomolecules (2 hours)
Metabolism and bioenergetics (2 hours)
Carbohydrates metabolism: glycolysis, pentose phosphate pathway, glycogen, gluconeogenesis, synthesis of lactose (4 hours)
TCA cycle and oxidative phosphorylation (2 hours)
Fatty acid catabolism and lipid biosynthesis. Ketone bodies. Cholesterol metabolism (4 hours)
Amino acid metabolism, ammonia metabolism and urea cycle (3 hours)
Nucleotides: purines and pyrimidines metabolism (2 hours)
Metabolic integration and intra-extra cellular regulation (2 hours)
Nucleic acids and biological function. Genetic code. DNA replication, transcription, gene expression and protein synthesis (11 hours)
Rumen biochemistry (4 hours)
Lab technologies (2 hours)
Prerequisites for admission
In order to attend the course profitably the student should be endowed with basic knowledge of general, inorganic and organic chemistry. It is highly recommended to follow the course "Chemistry" held in the first semester. Alternatively, students can use any basic level chemistry textbook or on-line material.
Teaching methods
The course consists of classroom theoretical lectures supported by seminars and laboratory practice on main biochemical biochemical tecniques applied in analytical laboratories and specific topics of interest, with the aid of on-line scientific movies in order to describe the complex molecular events leading to nucleic acids and protein synthesis
Teaching Resources
Power Point slides on Ariel web-site (https://myariel.unimi.it)
One of the following textbooks:
D.L. Nelson M.M. Cox Introduzione alla biochimica di Lehninger (Zanichelli)
Campbell Farrell Biochimica (Edises)
One of the following textbooks:
D.L. Nelson M.M. Cox Introduzione alla biochimica di Lehninger (Zanichelli)
Campbell Farrell Biochimica (Edises)
Assessment methods and Criteria
The examination consists of a written test consisting in 4 open-ended questions, at least one for each of the following general subject: metabolism and its control, general biochemistry, molecular biology and the structure of a nucleic acid. Students must complete the test in 120 minutes. The overall final score of the test, beside correctness, considers completeness, clarity, synthesis and use of proper terminology. Grade are in thirtieths.
Exams are in number of 7 in the Academic year and take place in the months of January, February, April June, July, September and October, with the frequency set by the teaching board. Results will be communicated by SIFA.
Tests will be the same for attending and non-attending students.
Exams are in number of 7 in the Academic year and take place in the months of January, February, April June, July, September and October, with the frequency set by the teaching board. Results will be communicated by SIFA.
Tests will be the same for attending and non-attending students.
BIO/10 - BIOCHEMISTRY - University credits: 6
Lessons: 48 hours
Professor:
Nonnis Simona
Shifts:
Turno
Professor:
Nonnis SimonaProfessor(s)
Reception:
Monday 12.30-13.30
Department of Veterinary Medicine, Via dell'Università, 6 - 26900 Lodi, Italy