Analytic Chemistry Ii with Lab
A.Y. 2024/2025
Learning objectives
Theory module
Within this course the theoretical principles and instrumental methods relating to the main spectroscopic and separation analytical techniques will be illustrated. The resolution of common qualitative and quantitative problems will also be addressed, in particular regarding infrared spectroscopy, nuclear magnetic resonance and mass spectrometry techniques.
Laboratory module
Within the scope of the laboratory module, experimental procedures/ protocols, calibration methods as well as basic calculations (also using a spreadsheet) related to instrumental analytical chemistry will be described. The laboratory experiments will focus on instrumental spectroscopic (atomic and molecular spectroscopies) and chromatographic (HPLC and GC) methods for the qualitative and quantitative determination of unknown samples.
Within this course the theoretical principles and instrumental methods relating to the main spectroscopic and separation analytical techniques will be illustrated. The resolution of common qualitative and quantitative problems will also be addressed, in particular regarding infrared spectroscopy, nuclear magnetic resonance and mass spectrometry techniques.
Laboratory module
Within the scope of the laboratory module, experimental procedures/ protocols, calibration methods as well as basic calculations (also using a spreadsheet) related to instrumental analytical chemistry will be described. The laboratory experiments will focus on instrumental spectroscopic (atomic and molecular spectroscopies) and chromatographic (HPLC and GC) methods for the qualitative and quantitative determination of unknown samples.
Expected learning outcomes
Theory module
At the end of the course the student will know the main spectroscopic and chromatographic techniques and will be able to solve small qualitative and quantitative analytical problems by using the most suitable instrumental technique. The student will also be able to identify the structure of simple organic compounds by studying the relative IR, NMR and MS spectra.
Laboratory module
Ability in choosing and utilising the most suitable instrumental analytical spectroscopic and/or chromatographic technique for the determination of unknown samples, use of the most common calibration methods for quantitative determinations and processing of related data (also with spreadsheet). Acquisition of the ability to work with a group of colleagues.
At the end of the course the student will know the main spectroscopic and chromatographic techniques and will be able to solve small qualitative and quantitative analytical problems by using the most suitable instrumental technique. The student will also be able to identify the structure of simple organic compounds by studying the relative IR, NMR and MS spectra.
Laboratory module
Ability in choosing and utilising the most suitable instrumental analytical spectroscopic and/or chromatographic technique for the determination of unknown samples, use of the most common calibration methods for quantitative determinations and processing of related data (also with spreadsheet). Acquisition of the ability to work with a group of colleagues.
Lesson period: First semester
Assessment methods: Esame
Assessment result: voto verbalizzato in trentesimi
Single course
This course can be attended as a single course.
Course syllabus and organization
Single session
Responsible
Lesson period
First semester
Prerequisites for admission
Fundamentals of General Chemistry and stoichiometry, Organic Chemistry I
Assessment methods and Criteria
-The laboratory module test (5 points): Written test 1 hour long including 2 exercises on preparation/dilution of standard solutions and/or the application of the Beer Equation and 3 questions/exercises based on exercises helded in class and laboratory experiments
- Written Theoretical module test:
4 questions of short solution which, only if correctly solved, allow to pass to the second part
5/6 questions on theory and spectra interpretation (IR, NMR, MS)
The two tests will be held in different day but they will be assessed with a single mark deriving from the sum of the single marks
- Written Theoretical module test:
4 questions of short solution which, only if correctly solved, allow to pass to the second part
5/6 questions on theory and spectra interpretation (IR, NMR, MS)
The two tests will be held in different day but they will be assessed with a single mark deriving from the sum of the single marks
Modulo: Laboratorio di Chimica Analitica II
Course syllabus
Security and lab practices information.
Basic calculation on preparation/dilution of solutions in molar and ppm scales.
Use of spreadsheet for analytical data processing.
Qualitative and quantitative experiences on the main spectroscopic (AAS, AES, UV-Vis, IR, fluorescence) and chromatographic (GC, HPLC) techniques presented during the lectures.
Basic calculation on preparation/dilution of solutions in molar and ppm scales.
Use of spreadsheet for analytical data processing.
Qualitative and quantitative experiences on the main spectroscopic (AAS, AES, UV-Vis, IR, fluorescence) and chromatographic (GC, HPLC) techniques presented during the lectures.
Teaching methods
Practical numerical exercises in classroom and laboratory experiences based on theoretical lectures
Teaching Resources
Holler, Skoog, Crouch "Chimica Analitica Strumentale" EdiSES, VCH;
Lecture notes/slides
Lecture notes/slides
Modulo: Chimica Analitica II
Course syllabus
Principles of spectrophotometric and photoelectronic methods. Atomic adsorption and emission. Molecular spectroscopy: UV-vis, IR and Raman, fluorescence and phosphorescence.
Mass spectrometry.
Principles and methods of chromatographic techniques (GC and HPLC).
Set-up of qualitative and quantitative analyses.
Exercises on structure disclosing based on IR, UV and MS spectra. Practices of theoretical concepts. Numerical exercises on quantitative analyses involving spectroscopic and chromatographic techniques.
Nuclear Magnetic Resonance: monodimensional 1H- and 13C-NMR techniques. Spectrum parameter analyses: chemical shift and coupling constant. Overhauser effect.
Mass spectrometry.
Principles and methods of chromatographic techniques (GC and HPLC).
Set-up of qualitative and quantitative analyses.
Exercises on structure disclosing based on IR, UV and MS spectra. Practices of theoretical concepts. Numerical exercises on quantitative analyses involving spectroscopic and chromatographic techniques.
Nuclear Magnetic Resonance: monodimensional 1H- and 13C-NMR techniques. Spectrum parameter analyses: chemical shift and coupling constant. Overhauser effect.
Teaching methods
Lessons and interactive discussion
Resolution of IR, NMR, MS spectra.
Resolution of IR, NMR, MS spectra.
Teaching Resources
- Holler, Skoog, Crouch "Chimica Analitica Strumentale" EdiSES, VCH;
- R.M. Silverstein, F.X. Webster, Identificazione spettroscopica di composti organici, Ed. CEA,
-A.Randazzo "Guida pratica all'interpretazione di spettri NMR" Loghia Ed
- lecture notes and slides.
- R.M. Silverstein, F.X. Webster, Identificazione spettroscopica di composti organici, Ed. CEA,
-A.Randazzo "Guida pratica all'interpretazione di spettri NMR" Loghia Ed
- lecture notes and slides.
Modulo: Chimica Analitica II
CHIM/01 - ANALYTICAL CHEMISTRY - University credits: 6
Lessons: 48 hours
Professor:
Prati Laura
Shifts:
Turno
Professor:
Prati Laura
Modulo: Laboratorio di Chimica Analitica II
CHIM/01 - ANALYTICAL CHEMISTRY - University credits: 6
Practicals: 40 hours
Laboratories: 40 hours
Lessons: 8 hours
Laboratories: 40 hours
Lessons: 8 hours
Professors:
Giannini Clelia, Guglielmi Vittoria
Shifts:
Professors:
Giannini Clelia, Guglielmi Vittoria
Corso A
Professor:
Guglielmi VittoriaCorso B
Professor:
Guglielmi VittoriaEducational website(s)
Professor(s)
Reception:
By appointment
Department of Chemistry, via Golgi 19 - Milano