Cellular Regeneration Techniques
A.Y. 2023/2024
Learning objectives
The course will provide the students with a detailed knowledge of the molecular and cellular principles that regulate embryonic development. This knowledge will be extended to the biology of stem cells both tissue specific and embryonic. The course will include some information on the most recent technologies of cell conversion. Finally, the possible therapeutic application of these cells will be critically assessed.
Expected learning outcomes
At the end of the course, the student acquires a solid knowledge of the molecular and cellular principles controlling embryonic development. This knowledge finds applications to the biology of embryonic and adult stem cells, as well as to the most recent technologies of cell conversion, with their possible therapeutic applications.
Lesson period: First semester
Assessment methods: Esame
Assessment result: voto verbalizzato in trentesimi
Single course
This course cannot be attended as a single course. Please check our list of single courses to find the ones available for enrolment.
Course syllabus and organization
Single session
Responsible
Lesson period
First semester
Course syllabus
Unit 1: Regenerative medicine, biological principles and laboratory procedures
Stem cells: biological principles 2 hours
Embryonic stem cells (ESC): origins and biological characteristics 2 hours
The molecular mechanisms that regulate pluripotency of ESC 2 hours
Cellular reprogramming 2 hours
iPS cells 2 hours
Cell conversion 2 hours
Mesenchymal stem cells 2 hours
Intestinal stem cells 2 hours
Neural stem cells 2 hours
Student presentations on topics of their choice and discussion 6 hours
Unit 2: Molecular Embryology
Key molecules in early embryo development
Trofectoderm and inner cell mass. Transcription factors controlling cell plasticity
Imprinting and epigenetic regulation
Maternal and paternal contribution to the mitotic spindle, centromere and centrioles
Cytoplasm compartimentalization
Telomerase and cell death
Epigenetics and cell fate definition
Developmental cues, gradients, morphogenesis
Genome editing (siRNA, ZFN, Talens, Crispr/Cas)
Stem cells: biological principles 2 hours
Embryonic stem cells (ESC): origins and biological characteristics 2 hours
The molecular mechanisms that regulate pluripotency of ESC 2 hours
Cellular reprogramming 2 hours
iPS cells 2 hours
Cell conversion 2 hours
Mesenchymal stem cells 2 hours
Intestinal stem cells 2 hours
Neural stem cells 2 hours
Student presentations on topics of their choice and discussion 6 hours
Unit 2: Molecular Embryology
Key molecules in early embryo development
Trofectoderm and inner cell mass. Transcription factors controlling cell plasticity
Imprinting and epigenetic regulation
Maternal and paternal contribution to the mitotic spindle, centromere and centrioles
Cytoplasm compartimentalization
Telomerase and cell death
Epigenetics and cell fate definition
Developmental cues, gradients, morphogenesis
Genome editing (siRNA, ZFN, Talens, Crispr/Cas)
Prerequisites for admission
Courses of Histology, Anatomy, Physiology and Embryology
Teaching methods
Lectures, student presentations and practical classes in the lab
Teaching Resources
Handouts and updated scientific publications available to the students
Assessment methods and Criteria
The examination takes place as a single written examination of 30 multiple-choice questions (4 answers) with the possibility of choosing only one correct answers.
Exam are in number of 8 in the Academic year and take place in the months of January, February, April, June, July, September, October and December. There are no additional calls for out-of-course. To take the exam, it is essential to register for the exam through the ONLINE SIFA service of the University.
Exam are in number of 8 in the Academic year and take place in the months of January, February, April, June, July, September, October and December. There are no additional calls for out-of-course. To take the exam, it is essential to register for the exam through the ONLINE SIFA service of the University.
Unita' didattica: Embriologia molecolare, dalla cellula all'apparato
VET/01 - VETERINARY ANATOMY - University credits: 3
Practicals: 12 hours
Lessons: 12 hours
Lessons: 12 hours
Professors:
Brevini Tiziana, Pennarossa Georgia
Unita' didattica: Medicina rigenerativa: principi biologici e procedure di laboratorio
VET/01 - VETERINARY ANATOMY - University credits: 4
Lessons: 24 hours
Professor:
Brevini Tiziana
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Professor(s)