Diagnostic Techniques in Clinical Biochemistry, Molecular Biology and Clinical Pathology

Module of: CLINICAL BIOCHEMISTRY AND CLINICAL MOLECULAR BIOLOGY
1) Hormonology and related tests:
- General concepts (synthesis, release, action, receptor mechanisms, etc.)
- Hypothalamic hormones (TRH, etc.)
- Hormones g. anterior pituitary [TSH, FSH, LH, prolactin, growth hormone (GH), adenocorticotropic hormone (ACTH)]
- Hormones g. posterior pituitary (ADH and oxytocin)
- Thyroid hormones
- Parathyroid hormone (PTH)
- Adrenal cortical hormones (aldosterone, cortisol, DHEA)
- Adrenal medullary hormones
- Ovarian hormones (estrogens, progesterone, activine, inhibine, relaxin)
- Testosterone
- Gastro-intestinal hormones (gastrin, ghrelin, secretin, GIP)
- Renal hormones (vitamin D, erythropoietin, renin-angiotensin system, aldosterone)
- Hepatic hormones (IGF 1 and 2)
- Hormones of adipose tissue (adiponectin, leptin, resistin, TNFα)
- Catecholamines and serotonin
2) Laboratory and pituitary alterations (excess and deficiency of GH, hyperprolactinemia, ACTH and gonadotropin alterations)
3) Laboratory and disorders of the adrenal gland (hypofunction, hyperfunction, adrenogenital syndrome, hyperaldosteronism)
4) Laboratory and reproductive disorders (alterations of male development - hypogonadism, development of the female reproductive system and its abnormalities, male and female infertility)
5) The laboratory in the management of monoclonal gammopathies
6) General principles and optimization of the clinical use of biomarkers in oncology
7) Laboratory in renal disease:
- Glomerular damage markers and evaluation of glomerular filtration rate
- Tubular damage markers
8) Laboratory and diabetes mellitus (type I, type II, gestational and complication monitoring)
9) Laboratory and risk of cardiovascular disease (lipoproteins, apoproteins, C-reactive protein, homocysteine)
10) Laboratory in the diagnosis of acute coronary syndrome and in the evaluation of cardiac function
11) Laboratory in disorders of hydro-electrolyte balance and acid-base metabolism
12) Laboratory and hepatopathies
- Markers of liver function
- Markers of liver damage (acute, chronic, fibrosis, cirrhosis)
- Cholestasis markers
13) Laboratory in gastro-intestinal and pancreatic diseases (celiac disease, IBD / IBS, pancreatitis, evaluation of exocrine pancreatic function)
14) Laboratory in bone diseases:
- Alterations in mineral metabolism
- Alterations in regulatory hormones (PTH, vitamin D, FGF23, calcitonin)
- Alterations in bone remodeling
15) Clinical biochemistry of hemoglobin, bilirubin, iron, transferrin saturation, ferritin
16) Laboratory for alterations of iron metabolism
17) Biochemical monitoring of drugs:
- General pharmacokinetic concepts
- Antiepileptic drugs
- Antibiotics (aminoglycosidics, vancomycin, chloramphenicol)
- Antineoplastics and immunosuppressants (methotrexate, busulfan, tacrolimus, ciclosporin, sirolimus, everolimus, mycophenolic acid)
- Other drugs (digoxin, lithium, theophylline)
18) Mass spectrometry in clinical diagnostics
19) Pharmacogenetics with specific examples (cytochromes)

∙ The blood count
∙ Laboratory and coagulation
∙ The chemical-physical and morphological examination of the urine
∙ Stool examination
∙ Laboratory and autoimmune diseases
∙ Synovial fluid diagnostics