Molecular Microbiology
A.Y. 2021/2022
Learning objectives
Provide molecular tools for a comprehensive knowledge of veterinary microbiology and tools for understanding the utility of laboratory rodents as spontaneous or genetically modified disease models.
Expected learning outcomes
A) Theoretical learning
Gain knowledge on:
- Molecular mechanisms of interaction between micrrorganisms (bacteria and viruses) and the animal host.
-Major methods to study virulence factors and strategies of antibiotic resistance and host immune system evasion.
Spontaneous, induced and genetically modified animal models and of their most common experimental applications, of the laboratory animal environment, health monitoring and strain phenotyping.
B) Practical learning
Know how to plan and perform an experiment of gene expression (from biopsy to quantification of a selected molecule).
Know how to use bioinformatic tools to interpret results of genomics and proteomic experiments.
Knowledge of histomorphology of tissue lesions of major relevant diseases in laboratory animals (major focuns on dogs, mice and rats).
Gain knowledge on:
- Molecular mechanisms of interaction between micrrorganisms (bacteria and viruses) and the animal host.
-Major methods to study virulence factors and strategies of antibiotic resistance and host immune system evasion.
Spontaneous, induced and genetically modified animal models and of their most common experimental applications, of the laboratory animal environment, health monitoring and strain phenotyping.
B) Practical learning
Know how to plan and perform an experiment of gene expression (from biopsy to quantification of a selected molecule).
Know how to use bioinformatic tools to interpret results of genomics and proteomic experiments.
Knowledge of histomorphology of tissue lesions of major relevant diseases in laboratory animals (major focuns on dogs, mice and rats).
Lesson period: First semester
Assessment methods: Esame
Assessment result: voto verbalizzato in trentesimi
Single course
This course cannot be attended as a single course. Please check our list of single courses to find the ones available for enrolment.
Course syllabus and organization
Single session
Responsible
Lesson period
First semester
During the emergency teaching phase, the program of the course will be maintained as possible but modified to better adapt to on line teaching methodologies. More specific information on the delivery models of training and teaching activities for the academic year 2021/2022 will be provided over the coming months, based on the evolution of public health situation and modified according with the guidelines that will be published by UNIMI a accordingly.
Teaching Methods:
Lectures will be delivered online by Teams videoconferences according with the course schedule. Lectures will be video recorded in parallel or provided in the Ariel web site of each professor.
Practicals will be performed according with the changing guidelines based on public health situation. Practicals at the microscope will be substituted by online practical with digitally scanned slides.
Teaching material:
Teaching material resources (power point, pdf, videos and scientific papers) will be made available in the Ariel web sites of the professors.
Assessment:
The exam will consist of a written exam composed of only multiple choice questions delivered through the online Forms platform with vigilance via the Teams platforms. Links will be provided after the closure of the subscriptions and sent to the student representatives. Questions will be assembled exclusively from the teaching material provided in Ariel by each professor.
Teaching Methods:
Lectures will be delivered online by Teams videoconferences according with the course schedule. Lectures will be video recorded in parallel or provided in the Ariel web site of each professor.
Practicals will be performed according with the changing guidelines based on public health situation. Practicals at the microscope will be substituted by online practical with digitally scanned slides.
Teaching material:
Teaching material resources (power point, pdf, videos and scientific papers) will be made available in the Ariel web sites of the professors.
Assessment:
The exam will consist of a written exam composed of only multiple choice questions delivered through the online Forms platform with vigilance via the Teams platforms. Links will be provided after the closure of the subscriptions and sent to the student representatives. Questions will be assembled exclusively from the teaching material provided in Ariel by each professor.
Course syllabus
The number of laboratory animal models in comparative research has expanded in quantity thanks to novel techniques of rearing. Objective of the course are to provide the students of biotechnological sciences with insights to the adequate approach to the study and characterization of infectious agents and their relevance for laboratory animals The application of biotechnologies have permitted the creation of transgenic animals (GEM genetically engineered mice). New microbiological statuses have developed and their recognition and approach have changed in the last years as an example as the microbiological status of laboratory animals can interfere with results of the experiments, some agents on the contrary can not be eradicated and need to be acknowledged as possible interferences. Scientific personnel of various disciplines are involved in the production and study of laboratory animals and their microbiological status which significance is completely different from other animals. These different professions need to integrate their specialties in a team based collaboration. The course I organized in 3 Units: a) Molecular aspects of the interaction of microorganisms and hosts b) Molecular basis of microbial pathogenicity c) Experimental pathology . Units a and b will be held in Italian, unit c in English
Module: 'H52-66-A' - 'Teaching Unit: Molecular aspects of the interaction of microorganisms and hosts
Module Program
Theoretical classes:
General aspects of microorganism-host interactions
Molecular aspects of microorganism-host interactions
Receptors: the antigen recognition molecules
Bacteria-host interactions
Virus-host interactions
Practical classes:
Isolation and characterization of pattern recognition receptors
Program for not attending students :
Theoretical classes:
General aspects of microorganism-host interactions
Molecular aspects of microorganism-host interactions
Receptors: the antigen recognition molecules
Bacteria-host interactions
Virus-host interactions
Practical classes:
Isolation and characterization of pattern recognition receptors
Program's information:
Study material will be provided by the teacher in class.
Program's information for not attending students:
Study material will be provided by the teacher in class.
Module: 'H52-66-B' - "Teaching Unit: Molecular basis of microbial pathogenicity"
Program
Theoretical part:
Course Introduction
genetic mechanisms in pathogenicity
The virulence factors: techniques for the 'identification and their characteristics
Virulence and its regulation: secretion systems and quorum sensing
molecular strategies for evasion of the host's immune system
antibiotics and microbial resistance
pathogenic microorganisms in veterinary field
New strategies to control the pathogenicity : the microbiome and its modulation
EXERCISES:
bioinformatics methods applied to the study of microbial pathogenicity
SEMINARS:
the seminars will be conducted by internal researchers and / or by external collaborators working in the microbiological and related field.
[Program for not attending students with reference to descriptor 1 and 2]:
Teaching unit: Molecular basis of microbial pathogenicity:
Theoretical part:
Course Introduction
genetic mechanisms in pathogenicity
The virulence factors: techniques for the 'identification and their characteristics
Virulence and its regulation: secretion systems and quorum sensing
molecular strategies for evasion of the host's immune system
antibiotics and microbial resistance
pathogenic microorganisms in veterinary field
New strategies to control the pathogenicity : the microbiome and its modulation
EXERCISES:
bioinformatics methods applied to the study of microbial pathogenicity
SEMINARS:
the seminars will be conducted by internal researchers and / or by external collaborators working in the microbiological and related field.
Teaching material:
Readings and teaching material for the exam are provided by the teacher in class (copies, pdf,ppt)
Module: 'H52-66-C' - 'Unit: Experimental Pathology'.
Presentation:
The number of laboratory animal models in comparative research has expanded in quantity thanks to the application of biotechnologies to laboratory rodents that have permitted the creation of transgenic animals. Scientific personnel of various disciplines are involved in the production and study of laboratory animal models. These different professions need to integrate their specialties in team based collaboration.
COURSE OBJECTIVES:
Objective of the course is to provide the students of biotechnological sciences with insights to the adequate approach to the comprehension of most utilized animal models . The course will illustrate and explain major comparative spontaneous and experimental models of disease from the perspective of pathology
ORGANIZATION OF THE COURSE:
The course will be held by the responsible aided by pathologists working in different fields with laboratory animals (academia, private research centers, pharmaceutical industry, contract laboratories).
LECTURES:
· The role of the veterinary pathologist in laboratory animal studies
· Most utilized animal models.
· Transgenic mice: production and characterization.
· Transgenic mouse models of infectious diseases.
· Principles of phenotyping of transgenic mice
· Nomenclature of mouse strains and genetically modified mice.
· Main spontaneous diseases (environmental, aging, infectious, neoplastic) in laboratory rodents
· Main diseases of rodent laboratory strains
· Immune deficient mice: infectious diseases
Module: 'H52-66-A' - 'Teaching Unit: Molecular aspects of the interaction of microorganisms and hosts
Module Program
Theoretical classes:
General aspects of microorganism-host interactions
Molecular aspects of microorganism-host interactions
Receptors: the antigen recognition molecules
Bacteria-host interactions
Virus-host interactions
Practical classes:
Isolation and characterization of pattern recognition receptors
Program for not attending students :
Theoretical classes:
General aspects of microorganism-host interactions
Molecular aspects of microorganism-host interactions
Receptors: the antigen recognition molecules
Bacteria-host interactions
Virus-host interactions
Practical classes:
Isolation and characterization of pattern recognition receptors
Program's information:
Study material will be provided by the teacher in class.
Program's information for not attending students:
Study material will be provided by the teacher in class.
Module: 'H52-66-B' - "Teaching Unit: Molecular basis of microbial pathogenicity"
Program
Theoretical part:
Course Introduction
genetic mechanisms in pathogenicity
The virulence factors: techniques for the 'identification and their characteristics
Virulence and its regulation: secretion systems and quorum sensing
molecular strategies for evasion of the host's immune system
antibiotics and microbial resistance
pathogenic microorganisms in veterinary field
New strategies to control the pathogenicity : the microbiome and its modulation
EXERCISES:
bioinformatics methods applied to the study of microbial pathogenicity
SEMINARS:
the seminars will be conducted by internal researchers and / or by external collaborators working in the microbiological and related field.
[Program for not attending students with reference to descriptor 1 and 2]:
Teaching unit: Molecular basis of microbial pathogenicity:
Theoretical part:
Course Introduction
genetic mechanisms in pathogenicity
The virulence factors: techniques for the 'identification and their characteristics
Virulence and its regulation: secretion systems and quorum sensing
molecular strategies for evasion of the host's immune system
antibiotics and microbial resistance
pathogenic microorganisms in veterinary field
New strategies to control the pathogenicity : the microbiome and its modulation
EXERCISES:
bioinformatics methods applied to the study of microbial pathogenicity
SEMINARS:
the seminars will be conducted by internal researchers and / or by external collaborators working in the microbiological and related field.
Teaching material:
Readings and teaching material for the exam are provided by the teacher in class (copies, pdf,ppt)
Module: 'H52-66-C' - 'Unit: Experimental Pathology'.
Presentation:
The number of laboratory animal models in comparative research has expanded in quantity thanks to the application of biotechnologies to laboratory rodents that have permitted the creation of transgenic animals. Scientific personnel of various disciplines are involved in the production and study of laboratory animal models. These different professions need to integrate their specialties in team based collaboration.
COURSE OBJECTIVES:
Objective of the course is to provide the students of biotechnological sciences with insights to the adequate approach to the comprehension of most utilized animal models . The course will illustrate and explain major comparative spontaneous and experimental models of disease from the perspective of pathology
ORGANIZATION OF THE COURSE:
The course will be held by the responsible aided by pathologists working in different fields with laboratory animals (academia, private research centers, pharmaceutical industry, contract laboratories).
LECTURES:
· The role of the veterinary pathologist in laboratory animal studies
· Most utilized animal models.
· Transgenic mice: production and characterization.
· Transgenic mouse models of infectious diseases.
· Principles of phenotyping of transgenic mice
· Nomenclature of mouse strains and genetically modified mice.
· Main spontaneous diseases (environmental, aging, infectious, neoplastic) in laboratory rodents
· Main diseases of rodent laboratory strains
· Immune deficient mice: infectious diseases
Prerequisites for admission
Undergraduate three year university programmes.
Recommended (not mandatory): Knowledge of basic anatomy, basic histology, basic physiology and basic laboratory methods.
Recommended (not mandatory): Knowledge of basic anatomy, basic histology, basic physiology and basic laboratory methods.
Teaching methods
Lectures consist of frontal teaching and seminars.
Practicals willb e delivered in the laboratory and at the multiheaded microscope or by discussion of scanned slides in class.
Practicals willb e delivered in the laboratory and at the multiheaded microscope or by discussion of scanned slides in class.
Teaching Resources
Study material will be provided to the students by the professors as pdf files videorecorded lectures and videos posted in the dedicated ARIEL web sites.
Assessment methods and Criteria
The exam will be written and delivered for all 3 modules contemporarily. Questions of the experimental pathology module will be in English. The exam will consist of a mixture of multiple choice questions, fill in the table, fill sentencens, short essay type questions and more.
Enrollment trough SIFA is mandatory to access the exam.
Enrollment trough SIFA is mandatory to access the exam.
Unit: Experimental Pathology
BIO/19 - MICROBIOLOGY
MED/04 - EXPERIMENTAL MEDICINE AND PATHOPHYSIOLOGY
MED/07 - MICROBIOLOGY AND CLINICAL MICROBIOLOGY
MED/04 - EXPERIMENTAL MEDICINE AND PATHOPHYSIOLOGY
MED/07 - MICROBIOLOGY AND CLINICAL MICROBIOLOGY
Practicals: 12 hours
Lessons: 18 hours
Lessons: 18 hours
Professor:
Roccabianca Paola
Unita' didattica: Aspetti molecolari dell'interazione microrganismo-ospite
BIO/19 - MICROBIOLOGY
MED/04 - EXPERIMENTAL MEDICINE AND PATHOPHYSIOLOGY
MED/07 - MICROBIOLOGY AND CLINICAL MICROBIOLOGY
MED/04 - EXPERIMENTAL MEDICINE AND PATHOPHYSIOLOGY
MED/07 - MICROBIOLOGY AND CLINICAL MICROBIOLOGY
Practicals: 12 hours
Lessons: 12 hours
Lessons: 12 hours
Professor:
Turin Lauretta
Unita' didattica: Basi molecolari della patogenicità microbica
BIO/19 - MICROBIOLOGY
MED/04 - EXPERIMENTAL MEDICINE AND PATHOPHYSIOLOGY
MED/07 - MICROBIOLOGY AND CLINICAL MICROBIOLOGY
MED/04 - EXPERIMENTAL MEDICINE AND PATHOPHYSIOLOGY
MED/07 - MICROBIOLOGY AND CLINICAL MICROBIOLOGY
Practicals: 12 hours
Lessons: 12 hours
Lessons: 12 hours
Professor:
Soggiu Alessio
Professor(s)
Reception:
Monday 9:00 a.m. - 1 p.m.
Polo Lodi